A new method for permeabilization of the plasma membrane of cultured mammalian cells .5. A standard procedure recommended for permeabilization by vortex-stirring with high molecular weight polyacrylic acid

被引：0

作者：

Shimizu, N ^{[1
]}

Kawazoe, Y ^{[1
]}

机构：

[1] NAGOYA CITY UNIV, FAC PHARMACEUT SCI, MIZUHO KU, NAGOYA, AICHI 467, JAPAN

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1996年 / 19卷 / 10期

关键词：

permeabilization; mammalian cell; polyacrylic acid; plasma membrane; vortex-stirring;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

As we previously reported, permeabilization of the plasma membrane was effectively achieved by vortex-stirring the mammalian cells in the presence of high molecular weight polyacrylic acid. Based on our present findings, as well as those of our previous studies, optimal conditions for permeabilization were considered in terms of the structure and dosage of a polymer, the constituent of the medium, the conditions of cells, vortex-stirring and post-vortex-stirring, etc. A standard procedure tentatively recommended is described.

引用

页码：1279 / 1284

页数：6

共 9 条

[1] A new method for permeabilization of the plasma membrane of cultured mammalian cells .7. Permeabilization of growing and resting cells by vortex-stirring with high molecular weight polyacrylic acid
Noda, Y
Shimizu, N
Kawazoe, Y
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1996, 19 (12) : 1565 - 1569
[2] Delicate and crucial pH dependence in permeabilization of cultured mammalian cells by vortex-stirring with a high molecular weight polyacrylic acid
Shimizu, N
Kawazoe, Y
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1996, 19 (11) : 1533 - 1535
[3] A new method for permeabilization of the plasma membrane of cultured mammalian cells .3. Internalization of fluorescent dextrans into cultured mammalian cells by vortex-stirring in the presence of high molecular weight polyacrylic acid
Shimizu, N
Kawazoe, Y
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1996, 19 (08) : 1023 - 1025
[4] A new method for permeabilization of the plasma membrane of cultured mammalian cells .2. Mechanism for potentiation of bleomycin cytotoxicity by vortex-stirring with high molecular weight polyacrylic acid
Shimizu, N
Kawazoe, Y
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1996, 19 (06) : 805 - 808
[5] A new method for permeabilization of the plasma membrane of cultured mammalian cells .4. Structural requirement for high molecular weight polymers for potentiation of bleomycin cytotoxicity toward mammalian cells under conditions of vortex-stirring
Shimizu, N
Nakamura, M
Azuchi, M
Okada, M
Kawazoe, Y
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1996, 19 (09) : 1126 - 1129
[6] A new method for permeabilization of the plasma membrane of cultured mammalian cells .6. Enhanced cytotoxicity of bleomycin toward leukemia P388 cells inoculated into the tail vein of mice together with high molecular weight polyacrylic acid - Similarity of blood stream to vortex-stirring
Ikeda, Y
Shimizu, N
Kawazoe, Y
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1997, 20 (01) : 25 - 27
[7] Internalization of non-permeant materials into cultured mammalian cells by vortex-stirring in the presence of a high molecular weight polyacrylic acid: Direct evidence of internalization and its dependence on the cell lines
Shimizu, N
Kawazoe, Y
Furuno, T
Nakanishi, M
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1997, 20 (07) : 727 - 731
[8] Enhanced cytotoxicity of alkyl viologens and N,N'-diamino analogs toward cultured murine leukemia L1210 cells under vortex-stirring with a high molecular weight polyacrylic acid
Shimizu, N
Wu, W
Kohara, A
Kohda, K
Suzuki, T
Kawazoe, Y
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1997, 20 (02) : 168 - 170
[9] POTENTIATION OF BLEOMYCIN CYTOTOXICITY IN CULTURED-MAMMALIAN-CELLS BY HIGH-MOLECULAR-WEIGHT POLYACRYLIC-ACID
SUZUKI, T
KOHDA, K
KAWAZOE, Y
ANTICANCER RESEARCH, 1990, 10 (04) : 1001 - 1004

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