Survival of frozen or vitrified bovine blastocysts produced in vitro in synthetic oviduct fluid

被引:31
|
作者
Dinnyes, A
Carolan, C
Lonergan, P
Massip, A
Mermillod, P
机构
[1] INRA,REPROD PHYSIOL UNIT,F-37380 NOUZILLY,FRANCE
[2] INST ANIM SCI,AGR BIOTECHNOL CTR,GODOLLO,HUNGARY
[3] CATHOLIC UNIV LEUVEN,VET UNIT,B-3000 LOUVAIN,BELGIUM
关键词
in vitro; bovine; embryo; cryopreservation; SOF;
D O I
10.1016/S0093-691X(96)00321-4
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Simplification of in vitro culture conditions offers the advantage of limiting problems due to variation in composition of biological fluids and co-culture with epithelial cells. The aim of this work was to study cryosurvival of bovine embryos produced under partially defined conditions. A conventional freezing technique and a vitrification method that allow for direct transfer of embryos after thawing were compared. Following IVM and IVF, zygotes were cultured in modified synthetic oviduct fluid (SOF), with 10% fetal calf serum (FCS) added 48 h post insemination, or, in the absence of FCS, in a humidified atmosphere of 5%O-2, 5%CO2, 90%N-2. Morphologically normal Day 8 and 9 blastocysts were classified into 3 groups and were cryopreserved. Group A were blastocysts (<150 mu m); Group B consisted of expanded blastocysts (>150 mu m); while Group C were hatching or hatched blastocysts. The freezing solution consisted of 1.36 M glycerol and 0.25 M sucrose in PBS. The vitrification solution consisted of 6.5 M glycerol and 6% bovine serum albumin in PBS (VS3a). Thawed embryos were cultured on granulosa cells and survival was defined as the re-expansion and maintenance of the blastocoel over 24 and 72 h, respectively. Survival rates following vitrification were similar or better than after freezing (Day 8 embryo survival rate at 72 h was 72 vs 57%). More advanced embryos of the same group seemed to survive better. Embryos cultured without serum survived cryopreservation significantly better than those produced with addition of serum at 48 h post insemination (86 vs 61% at 72 h; P<0.01). Hatched blastocysts survived freezing or vitrification well (80 to 100%). One calf was born following the transfer of 6 vitrified embryos. The results indicate that both freezing and vitrification methods are suitable for cryopreservation of bovine embryos produced in SOF.
引用
收藏
页码:1425 / 1439
页数:15
相关论文
共 50 条
  • [1] EFFECT OF SUGARS ADDITION ON THE SURVIVAL OF VITRIFIED BOVINE BLASTOCYSTS PRODUCED IN-VITRO
    SAITO, N
    IMAI, K
    TOMIZAWA, M
    [J]. THERIOGENOLOGY, 1994, 41 (05) : 1053 - 1060
  • [2] Factors affecting the survival of frozen thawed bovine in vitro produced blastocysts
    Gustafsson, H
    Larsson, B
    Shamsuddin, M
    Jaakma, U
    Emanúelson, U
    [J]. ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 2001, 14 (01): : 7 - 12
  • [3] Survival and viability of vitrified in vitro and in vivo produced ovine blastocysts
    Dattena, M
    Ptak, G
    Loi, P
    Cappai, P
    [J]. THERIOGENOLOGY, 2000, 53 (08) : 1511 - 1519
  • [4] IMPROVED SURVIVAL TO ONE-STEP REHYDRATION OF VITRIFIED-WARMED VERSUS FROZEN-THAWED IN VITRO-PRODUCED BOVINE BLASTOCYSTS
    Trigal, B.
    Gomez, E.
    Caamano, J. N.
    Munoz, M.
    Correia, E.
    Martin, D.
    Carrocera, S.
    Diez, C.
    [J]. REPRODUCTION FERTILITY AND DEVELOPMENT, 2013, 25 (01) : 189 - 189
  • [5] Determinants Survival Aspects of Vitrified In Vitro Produced Bovine Embryos
    Sudano, Mateus J.
    Paschoal, Daniela M.
    Rascado, Tatiana S.
    Guastali, Midyan D.
    Maziero, Rosiara R.
    Magalhaes, Luis C. O.
    Crocomo, Leticia F.
    Lima-Neto, Joao F.
    Martins, Alicio, Jr.
    Machado, Rui
    Landim-Alvarenga, Fernanda C.
    [J]. BIOLOGY OF REPRODUCTION, 2011, 85
  • [6] GLOBAL GENE EXPRESSION PATTERNS OF FRESH AND VITRIFIED IN VITRO-PRODUCED BOVINE BLASTOCYSTS
    Sudano, M. J.
    Caixeta, E. S.
    Paschoal, D. M.
    Rascado, T. S.
    Crocomo, L. F.
    Maziero, R. R.
    Magalhaes, L. C. O.
    Guastali, M. D.
    Vergara, L. E.
    Monteiro, B. A.
    Martins, A., Jr.
    Machado, R.
    Buratini, J.
    Landin-Alvarenga, F. C.
    [J]. REPRODUCTION FERTILITY AND DEVELOPMENT, 2013, 25 (01) : 187 - 188
  • [7] Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts
    Zongliang Jiang
    Patrick Harrington
    Ming Zhang
    Sadie L. Marjani
    Joonghoon Park
    Lynn Kuo
    Csaba Pribenszky
    Xiuchun (Cindy) Tian
    [J]. Scientific Reports, 6
  • [8] Higher survival rate of vitrified and thawed in vitro produced bovine blastocysts following culture in defined medium supplemented with β-mercaptoethanol
    Nedambale, Tshimangadzo Lucky
    Du, Fuliang
    Yang, Xiangzhong
    Tian, Xiuchun Cindy
    [J]. ANIMAL REPRODUCTION SCIENCE, 2006, 93 (1-2) : 61 - 75
  • [9] Direct in-straw rehydration after thawing of vitrified in vitro produced bovine blastocysts
    Vajta, G
    Holm, P
    Greve, T
    Callesen, H
    [J]. VETERINARY RECORD, 1995, 137 (26) : 672 - 672
  • [10] Effects of High Hydrostatic Pressure on Expression Profiles of In Vitro Produced Vitrified Bovine Blastocysts
    Jiang, Zongliang
    Harrington, Patrick
    Zhang, Ming
    Marjani, Sadie L.
    Park, Joonghoon
    Kuo, Lynn
    Pribenszky, Csaba
    Tian, Xiuchun
    [J]. SCIENTIFIC REPORTS, 2016, 6