Genetic determination and localization of multiple bacteriocins produced by Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431

被引：8
|
作者：
Aguilar-Galvez, Ana [1 ,2 ]
Dubois-Dauphin, Robin [2 ]
Campos, David [1 ]
Thonart, Philippe [2 ]
机构：
[1] Univ Nacl Agr La Molina, Inst Biotecnol, Lima, Peru
[2] Univ Liege, Gembloux Agro Bio Tech Unit Bioind, B-5030 Gembloux, Belgium
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2011年 / 20卷 / 02期
关键词：
Enterococcus faecium; Enterococcus mundtii; enterocin P; mundticin KS; genetic expression; SEC-DEPENDENT BACTERIOCIN; DRY-FERMENTED SAUSAGES; ESCHERICHIA-COLI; STRAINS; PEPTIDE; L50; IDENTIFICATION; EXPRESSION; P13;
D O I：
10.1007/s10068-011-0041-6
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Enterococcus faecium CWBI-B1430 and Enterococcus mundtii CWBI-B1431 from artisanal-produced Peruvian cheeses showed the presence of 4 putative bacteriocin genes: enterocin A, enterocin B, enterocin P, and mundticin KS. The multiple bacteriocin producer E. faecium CWBI-B1430 presented 1 plasmid of 34.6 kb, whereas E. mundtii CWBI-B1431 contained 1 plasmid of 11.0 kb. The structural gene responsible for mundticin KS production was located on 5.6 and 3.1 kb HindIII plasmid fragments. The reverse transcription-PCR analysis showed the expression of the bacteriocin genes enterocin A, enterocin B, and mundticin KS in E. faecium CWBI-B1430 and the bacteriocin genes enterocin P and mundticin KS in E. mundtii CWBI-B1431. To our knowledge, this is the first report of the expression of mundticin KS in E. faecium and enterocin P in E. mundtii.
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收藏
页码：289 / 296
页数：8
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