Functional activation of Gαq coupled with serotonin receptors in cerebral cortical membranes determined by antibody-capture scintillation proximity assay/[35S]GTPγS binding

被引：0

作者：

Odagaki, Yuji ^{[1
,2
]}

Toyoshima, Ryoichi ^{[1
]}

机构：

[1] Saitama Med Univ, Dept Psychiat, Moroyama, Saitama 3500495, Japan

[2] Moro Hosp, Moroyama, Saitama 3500495, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2012年 / 118卷

关键词：

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

引用

页码：179P / 179P

页数：1

共 41 条

[1] Functional activation of Gq coupled with muscarinic acetylcholine receptors in rat brain membranes: An antibody-capture scintillation proximity assay [35S]GTPγS binding
Odagaki, Yuji
Toyoshima, Ryoichi
JOURNAL OF PHARMACOLOGICAL SCIENCES, 2011, 115 : 190P - 190P
[2] Activation of Gq Proteins Coupled with 5-HT2 Receptors in Rat Cerebral Cortical Membranes Assessed by Antibody-Capture Scintillation Proximity Assay/[35S]GTPγS Binding
Odagaki, Yuji
Toyoshima, Ryoichi
PHARMACOLOGY, 2013, 92 (1-2) : 2 - 10
[3] The antibody-capture [35S]GTP-γS scintillation proximity assay:: a powerful emerging technique for analysis of GPCR pharmacology
DeLapp, NW
TRENDS IN PHARMACOLOGICAL SCIENCES, 2004, 25 (08) : 400 - 401
[4] Antibody-capture scintillation proximity assay/[35S]GTPyS binding to Gaq functionally coupled with M1 acetylcholine receptors and 5-HT2A receptors in rat brain membranes
Odagaki, Y.
Toyoshima, R.
INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY, 2012, 15 : 165 - 165
[5] A fully automated [35S]GTPγS scintillation proximity assay for the high-throughput screening of Gi-linked G protein-coupled receptors
Ferrer, M
Kolodin, GD
Zuck, P
Peltier, R
Berry, K
Mandala, SM
Rosen, H
Ota, H
Ozaki, S
Inglese, J
Strulovici, B
ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2003, 1 (02) : 261 - 273
[6] Dopamine-stimulated [35S] GTPγS binding to Gαq in rat brain membranes
Odagaki, Yuji
Kinoshita, Masakazu
Ota, Toshio
JOURNAL OF PHARMACOLOGICAL SCIENCES, 2016, 130 (03) : S98 - S98
[7] Use of the GTPγS ([35S]GTPγS and Eu-GTPγS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors
Strange, Philip G.
BRITISH JOURNAL OF PHARMACOLOGY, 2010, 161 (06) : 1238 - 1249
[8] Muscarinic acetylcholine receptor-mediated activation of Gq in rat brain membranes determined by guanosine-5'-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding using an anti-G protein scintillation proximity assay
Odagaki, Yuji
Toyoshima, Ryoichi
JOURNAL OF NEURAL TRANSMISSION, 2012, 119 (05) : 525 - 532
[9] Muscarinic acetylcholine receptor-mediated activation of Gq in rat brain membranes determined by guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding using an anti-G protein scintillation proximity assay
Yuji Odagaki
Ryoichi Toyoshima
Journal of Neural Transmission, 2012, 119 : 525 - 532
[10] Functional coupling between adenosine A1 receptors and G-proteins in rat and postmortem human brain membranes determined with conventional guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding or [35S]GTPγS/immunoprecipitation assay
Yuji Odagaki
Masakazu Kinoshita
Toshio Ota
J. Javier Meana
Luis F. Callado
Isao Matsuoka
Jesús A. García-Sevilla
Purinergic Signalling, 2018, 14 : 177 - 190

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