Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy

被引：55
|
作者：
Park, HyunJoo [1 ]
Hong, Sung-Hee [2 ]
Kim, Kyoohyun [1 ]
Cho, Shin-Hyeong [2 ]
Lee, Won-Ja [2 ]
Kim, Youngchan [1 ]
Lee, Sang-Eun [2 ]
Park, YongKeun [1 ]
机构：
[1] Korea Adv Inst Sci & Technol, Dept Phys, Taejon 305701, South Korea
[2] Korea Ctr Dis Control & Prevent, Korea Natl Inst Hlth, Div Malaria & Parasit Dis, Chungbuk 363951, South Korea
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
新加坡国家研究基金会;
关键词：
DIFFRACTION OPTICAL TOMOGRAPHY; RECENT INSIGHTS; PHASE; DYNAMICS; VOLUME; PATHOPHYSIOLOGY; ERYTHROCYTES; HEMOGLOBIN; MALARIA; DISEASE;
D O I：
10.1038/srep10827
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Babesia microti causes "emergency" human babesiosis. However, little is known about the alterations in B. microti invaded red blood cells (Bm-RBCs) at the individual cell level. Through quantitative phase imaging techniques based on laser interferometry, we present the simultaneous measurements of structural, chemical, and mechanical modifications in individual mouse Bm-RBCs. 3-D refractive index maps of individual RBCs and in situ parasite vacuoles are imaged, from which total contents and concentration of dry mass are also precisely quantified. In addition, we examine the dynamic membrane fluctuation of Bm-RBCs, which provide information on cell membrane deformability.
引用
收藏
页数：11
相关论文
共 34 条
  • [1] Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy
    HyunJoo Park
    Sung-Hee Hong
    Kyoohyun Kim
    Shin-Hyeong Cho
    Won-Ja Lee
    Youngchan Kim
    Sang-Eun Lee
    YongKeun Park
    Scientific Reports, 5
  • [2] Clustering of Red Blood Cells Using Digital Holographic Microscopy
    Jaferzadeh, K.
    Ahmadzadeh, E.
    Moon, I.
    Gholami, S.
    HOLOGRAPHY: ADVANCES AND MODERN TRENDS V, 2017, 10233
  • [3] 3-D quantitative measurements of individual human red blood cells from diabetic patients employing 3-D quantitative phase imaging
    Lee, SangYun
    Park, YongKeun
    2016 ASIA COMMUNICATIONS AND PHOTONICS CONFERENCE (ACP), 2016,
  • [4] Holographic intravital microscopy for 2-D and 3-D imaging intact circulating blood cells in microcapillaries of live mice
    Kyoohyun Kim
    Kibaek Choe
    Inwon Park
    Pilhan Kim
    YongKeun Park
    Scientific Reports, 6
  • [5] Holographic intravital microscopy for 2-D and 3-D imaging intact circulating blood cells in microcapillaries of live mice
    Kim, Kyoohyun
    Choe, Kibaek
    Park, Inwon
    Kim, Pilhan
    Park, YongKeun
    SCIENTIFIC REPORTS, 2016, 6
  • [6] Statistical Analysis of 3D Volume of Red Blood Cells with Different Shapes via Digital Holographic Microscopy
    Yi, Faliu
    Lee, Chung-Ghiu
    Moon, In-Kyu
    JOURNAL OF THE OPTICAL SOCIETY OF KOREA, 2012, 16 (02) : 115 - 120
  • [7] AI-based analysis of 3D position and orientation of red blood cells using a digital in-line holographic microscopy
    Kim, Youngdo
    Kim, Jihwan
    Seo, Eunseok
    Lee, Sang Joon
    BIOSENSORS & BIOELECTRONICS, 2023, 229
  • [8] Imaging Embryonic Stem Cell Dynamics Using Quantitative 3-D Digital Holographic Microscopy
    Anand, A.
    Chhaniwal, V. K.
    Javidi, B.
    IEEE PHOTONICS JOURNAL, 2011, 3 (03): : 546 - 554
  • [9] Automated counting of morphologically normal red blood cells by using digital holographic microscopy and statistical methods
    Moon, Inkyu
    Yi, Faliu
    OPTICS AND PHOTONICS FOR INFORMATION PROCESSING IX, 2015, 9598
  • [10] Recognition and classification of red blood cells using digital holographic microscopy and data clustering with discriminant analysis
    Liu, Ran
    Dey, Dipak K.
    Boss, Daniel
    Marquet, Pierre
    Javidi, Bahram
    JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2011, 28 (06) : 1204 - 1210
1234