Postmenopausal women experiencing health transitions can improve health-related quality of life through clinical health service use. The aim of this study was to investigate the factors affecting clinical preventive service use, focusing on a multi-dimensional approach among middle-aged postmenopausal women. This descriptive study is a secondary analysis of the seventh Korea National Health and Nutrition Examination Survey (KNHANESVII-1) in 2016. Among the 8150 participants, our analysis included 771 naturally menopausal women aged 40-65. National health insurance (OR = 1.659, 95% CI = 1.080-2.550), private health insurance (OR = 2.877, 95% CI = 1.665-4.971), needs for health service (OR = 2.363, 95% CI = 1.332-4.195), cardiovascular disease (OR = 1.570, 95% CI = 1.009-2.445), hospital admission (OR = 3.054, 95% CI = 1.298-7.184), smoking (OR = 0.262, 95% CI = 0.144-0.477), drinking (OR = 0.573, 95% CI = 0.335-0.979), and depression (OR = 0.535, 95% CI = 0.340-0.841) were associated with clinical preventive service use among middle-aged postmenopausal women. To promote clinical preventive service use among postmenopausal women, policies promoting health behavior expansion should be introduced and should consider the predictive variables revealed by this study.