Identification of molecular patterns and diagnostic biomarkers in juvenile idiopathic arthritis based on the gene expression of m6A regulators

The role of N-6-methyladenosine modification in immunity is increasingly being appreciated. However, the landscape of m(6)A regulators in juvenile idiopathic arthritis (JIA) is poorly understood. Thus, this study explored the impact of m(6)A modification and related lncRNAs in JIA immune microenvironment. Fourteen m(6)A regulators and eight lncRNAs were identified as potential diagnostic biomarkers for JIA. Two diagnostic models for JIA were also constructed. The putative molecular regulatory mechanism of FTO-mediated m(6)A modification in JIA was hypothesized. Three distinct m(6)A patterns mediated by 26 m(6)A regulators and three diverse lncRNA clusters mediated by 405 lncRNAs were thoroughly investigated. They exhibited dramatically diverse immune microenvironments and expression of HLA genes. The identification of two separate subtypes of enthesitis-related arthritis implies that our work may aid in the establishment of a more precise categorization system for JIA. m(6)A modification-related genes were obtained, and their underlying biological functions were explored. The m(6)Ascore system developed for individual JIA patients may be utilized to evaluate the immunological state or molecular pattern, thereby offering therapy recommendations. In short, through the investigation of the m(6)A regulators in JIA, the current work may contribute to our knowledge of the pathophysiology of JIA.