Vanillin production using Escherichia coli cells over-expressing isoeugenol monooxygenase of Pseudomonas putida

被引：50

作者：

Yamada, Mamoru ^{[1
]}

Okada, Yukiyoshi ^{[1
]}

Yoshida, Toyokazu ^{[1
]}

Nagasawa, Toru ^{[1
]}

机构：

[1] Gifu Univ, Dept Biomol Sci, Gifu 5011193, Japan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 04期

关键词：

double-bond cleavage; isoeugenol; isoeugenol monooxygenase; vanillin production;

D O I：

10.1007/s10529-007-9602-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The isoeugenol monooxygenase gene of Pseudomonas putida IE27 was inserted into an expression vector, pET21a, under the control of the T7 promoter. The recombinant plasmid was introduced into Escherichia coli BL21(DE3) cells, containing no vanillin-degrading activity. The transformed E. coli BL21(DE3) cells produced 28.3 g vanillin/l from 230 mM isoeugenol, with a molar conversion yield of 81% at 20 degrees C after 6 h. In the reaction system, no accumulation of undesired by-products, such as vanillic acid or acetaldehyde, was observed.

引用

页码：665 / 670

页数：6

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