Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography

Reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) have been prepared by "in-situ" copolymerization of the monomers in the presence of porogenic dilute, and protein A was immobilized on the continuous rods directly or through a 11 carbon atom space arm with molded synthetic methods. The properties of these two rods columns were characterized and the results showed that the medium with space arm had some extent hydrophobicity. The affinity column without space arm was used to determine the human immunoglobulin G (HIgG) in human serum. The correlative coefficient of the calibration curve was 0.999; The effect of the flow rate on the pressure of the continuous rods was also investigated. The subclasses of HIgG were separated by the use of a pH gradient.