Determination of polychlorinated dibenzo-p-dioxins and furans and co-polar polychlorinated biphenyls in fat food by isotope dilution high resolution gas chromatography/high resolution mass spectrometry

A sensitive and accurate method has been developed for the simultaneously determination of 17 2,3 7,8 -substituted congeners of polychlorinated dibenzo-p-dioxins and furans ( PCDD/Fs) and 12 co-polar polychlorinated biphenyls ( Co-PCBs) in fat food as fish sample using isotope dilution high resolution gas chromatography/high resolution mass spectro-metry (HRGC/HRMS). PCDD/Fs and Co-PCBs were extracted from the sample by soxhlet extraction, concentrated and purified as well as isolated by FMS Power Prep. Confirmative and quantitative analysis of PCDD/Fs at pg/g level was performed by HRGC/HRMS using multiple ion detection mode ( MID) and isotope dilution high resolution gas chromatography/high resolution mass spectrometry. The recoveries of 17 PCDDs/Fs and 12 Co-PCBs isotopically labelled standards were found to be in the range of 47% similar to 100%. The quantitative value of 17 PCDDs/Fs and 4 PCBs concentration in fish certified reference material ( CRM) was droped in the range of certified value. The recoveries of internal standards in 5 different real fish samples were 48% similar to 100%, RSD < 20%. And the precision of quantitation PCDDs/Fs and Co-PCBs in sleeve-fish was also determined, RSD were less than 16 % and 11%, respectively. This method is a good analysis system in confirmation and quatitation for simultaneous determination of PCDDs/Fs and PCBs.