NMR structural determination of dissolved O-acetylated galactoglucomannan isolated from spruce thermomechanical pulp

被引：136
|
作者：
Hannuksela, T
du Penhoat, CH
机构：
[1] Abo Akad Univ, Proc Chem Grp, FIN-20500 Turku, Finland
[2] Univ Grenoble 1, CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble 9, France
来源：
CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 02期
关键词：
galactans; galactoglucomannans; O-acetyl groups; spruce; TMP; xylans;
D O I：
10.1016/j.carres.2003.10.025
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Water-soluble O-acetylated galactoglucomannan (GGM) isolated from spruce thermomechanical pulp (TMP) by hotwater extraction was characterized by 1D and 2D (homo- and heteronuclear) NMR analysis. The backbone was found to consist of (1 --> 4)-linked mannopyranosyl and glucopyranosyl units in a ratio of 10:1.9-2.6. The mannopyranosyl units were acetylated at C-2 and C-3 with a degree of acetylation around 0.28-0.37 as determined by NMR. A slightly larger amount of 2-O-acetylated mannopyranosyl was detected when compared to the 3-O-acetylated component. Approximately every 10th mannopyranosyl unit was substituted at C-6 by a single alpha-galactopyranosyl unit. Fine structure determination based on sequence-specific chemical shift variations showed that the distribution of glycosyl residues is random. Small amounts of other minor polysaccharide species including xylans and galactans could also be identified by NMR. (C) 2003 Elsevier Ltd. All rights reserved.
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页码：301 / 312
页数：12
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