THE USE OF BLACK-BOX MODELLING IN BIOPROCESS SCALE-UP

Computer models of bioprocesses have become an essential tool in biotechnology. This paper describes various bioprocess scaleup releated problems. The problems at different scales are explained. In this paper the use of computer model in E. coli fermentation scale-up from shake flask to laboratory scale bioreactor is discussed. A black box modelling approach was used. Fermentation results have been visualized and discussed. The computer model created in Matlab environment was used for bioprocess behaviour prediction. Possible bioprocess scale-up software improvements and bioprocess optimization are discussed.