Agglutination study of poly(allyl-α-D-glucopyranose/styrene) latex particles in the presence of Concanavalin A

被引：5

作者：

Lee, JY ^{[1
]}

Cheong, IW ^{[1
]}

Kim, JH ^{[1
]}

机构：

[1] Yonsei Univ, Dept Chem Engn, Nanosphere Proc & Technol Lab, Seodaemoon Ku, Seoul 120749, South Korea

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2005年 / 41卷 / 2-3期

关键词：

polymer colloid; biosensor; glucose; concanavalin A; binding; agglutination;

D O I：

10.1016/j.colsurfb.2004.12.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Latex agglutination of the glucose-modified latex, which were synthesized by emulsion copolymerization of allyl-modified glucose and styrene monomers by specific binding interactions between Concanavalin A (Con A) and allyl-U-D-glucopyranose, were investigated. The surface of the glucose-modified latex was characterized by dye-partition method and the number of glucose was 1517 per latex particle. The average particle size and the polydispersity index of the latex were 78.3 and 1.005, respectively. Time-evolution adsorption behavior of various concentrations of the Con A and the consequent latex agglutination were studied by UV spectrophotometer at 540 nm and zeta-potential analyzer at the fixed latex concentration of 0.02 wt%. Specific binding between Con A and allyl-U-D-glucopyranose leaded the latex particles to coagulate by decreasing the electrostatic repulsion between the particles and mobility. (c) 2004 Published by Elsevier B.V.

引用

页码：203 / 208

页数：6