Cloning and Site-specific Mutagenesis of DS Enzyme

被引:0
|
作者
Yu, Hansong [1 ]
Qu, Shuang [1 ]
Wang, Yuhua [1 ]
Piao, Chunhong [1 ]
Liu, Junmei [1 ]
Hu, Yaohui [1 ]
机构
[1] JiLin Agriclutural Univ, Coll Food Sci & Engn, Changchun 130118, Peoples R China
关键词
Fermentation Engineering; Corynebacterium glutamicum; key enzyme of amino acid; GENE; SEQUENCE;
D O I
暂无
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
In order to obtain the higher activity DS enzyme through genetic engineering technology. The point mutation method is used to get a higher activity recombinant DS enzyme from Corynebacterium glutamicum. At last the point 183 was mutated from L to Q in LG-322. The mutation type is a fundermental of construct a genetic engineering which has high activity DS enzyme.
引用
收藏
页码:106 / 110
页数:5
相关论文
共 50 条
  • [1] SITE-SPECIFIC MUTAGENESIS BY ACETYLAMINOFLUORENE
    MORIYA, M
    WILL, S
    JOHNSON, F
    GROLLMAN, AP
    [J]. PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1987, 28 : 109 - 109
  • [2] RAPID SITE-SPECIFIC MUTAGENESIS IN PLASMIDS
    FOSS, K
    MCCLAIN, WH
    [J]. GENE, 1987, 59 (2-3) : 285 - 290
  • [3] RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS
    BALDRICH, M
    GOEBEL, W
    [J]. PROTEIN ENGINEERING, 1990, 3 (06): : 563 - 563
  • [4] SITE-SPECIFIC MUTAGENESIS - RETROSPECTIVE AND PROSPECTIVE
    SINGER, B
    ESSIGMANN, JM
    [J]. CARCINOGENESIS, 1991, 12 (06) : 949 - 955
  • [5] SITE-SPECIFIC MUTAGENESIS OF HUMAN FOLLISTATIN
    INOUYE, S
    GUO, YL
    LING, N
    SHIMASAKI, S
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (01) : 352 - 358
  • [6] Molecular cloning and site-specific mutagenesis of a gene involved arylsulfatase production in Campylobacter jejuni
    Yao, RJ
    Guerry, P
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (11) : 3335 - 3338
  • [7] SITE-SPECIFIC MUTAGENESIS - RESULTS, PROBLEMS AND PERSPECTIVES
    KAINA, B
    [J]. BIOLOGISCHES ZENTRALBLATT, 1980, 99 (05): : 513 - 531
  • [8] SITE-SPECIFIC MUTAGENESIS OF RuBP CARBOXYLASE/OXYGENASE
    Hanks, Joanna F.
    Somerville, Christopher R.
    McIntosh, Lee
    [J]. PLANT PHYSIOLOGY, 1984, 75 : 64 - 64
  • [9] Site-specific mutagenesis of human pancreatic lipase
    Lowe, ME
    [J]. LIPASES, PART A: BIOTECHNOLOGY, 1997, 284 : 157 - 170
  • [10] Focused rational iterative site-specific mutagenesis (FRISM): A powerful method for enzyme engineering
    Bao, Yuyan
    Xu, Yuanyuan
    Huang, Xiaoqiang
    [J]. MOLECULAR CATALYSIS, 2024, 553