Studies on wide-field-of-view multiphoton imaging using the flexible clinical multiphoton tomograph MPTflex®

Multiphoton imaging systems are capable of high-resolution 3-D image acquisition of deep tissue. A first commercially available CE-certified biomedical system for subcelluar resolution of human skin has been launched by JenLab company with the DermaInspect (R) in 2002. The demand for more flexibility caused the development of the MPTflex (R), which provides an increased flexibility and accessibility especially for clinical and cosmetic examinations. However the high resolution of clinical multiphoton tomographs are adherent with a small field-of-view (FOV) of about 360x360 mu m(2). Especially time-consuming is the relocation of areas of interest (AOI) like lesions, sweat glands or hair shafts during a multiphoton examination. This limitation can be be overcome by macroscopic large-area (wide-field-of-view) multiphoton tomography, which is tested first within this work.