APOBEC4, a new member of the AID/APOBEC family of polynucleotide (deoxy)cytidine deaminases predicted by computational analysis

被引：108

作者：

Rogozin, IB

Basu, MK

Jordan, IK

Pavlov, YI

Koonin, EV ^{[1
]}

机构：

[1] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA

[2] Univ Nebraska, Med Ctr, Eppley Inst Res Canc, Omaha, NE USA

[3] Univ Nebraska, Med Ctr, Dept Biochem & Mol Biol, Omaha, NE USA

[4] Univ Nebraska, Med Ctr, Dept Pathol & Microbiol, Omaha, NE USA

来源：

CELL CYCLE | 2005年 / 4卷 / 09期

关键词：

cytidine deaminase; DNA/RNA modification; phylogenetic analysis; editing enzyme; innate immunity; APOBEC1;

D O I：

10.4161/cc.4.9.1994

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Using iterative database searches, we identified a new subfamily of the AID/APOBEC family of RNA/DNA editing cytidine deaminases. The new subfamily, which is represented by readily identifiable orthologs in mammals, chicken, and frog, but not fishes, was designated APOBEC4. The zinc-coordinating motifs involved in catalysis and the secondary structure of the APOBEC4 deaminase domain are evolutionarily conserved, suggesting that APOBEC4 proteins are active polynucleotide ( deoxy) cytidine deaminases. In reconstructed maximum likelihood phylogenetic trees, APOBEC4 forms a distinct clade with a high statistical support. APOBEC4 and APOBEC1 are joined in a moderately supported cluster clearly separated from AID, APOBEC2 and APOBEC3 subfamilies. In mammals, APOBEC4 is expressed primarily in testis which suggests the possibility that it is an editing enzyme for mRNAs involved in spermatogenesis.

引用

页码：1281 / 1285

页数：5