Participation of a stromal carbonic anhydrase and Rubisco in a thylakoid-associated multienzyme complex isolated from soybean and poplar

被引：0
|
作者：
Lazova, Galia N. [1 ]
Stemler, Alan J. [2 ]
机构：
[1] Bulgarian Acad Sci, Acad M Popov Inst Plant Physiol, BU-1113 Sofia, Bulgaria
[2] Univ Calif Davis, Plant Biol Sect, Davis, CA 95616 USA
来源：
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES | 2008年 / 61卷 / 05期
关键词：
carbonic anhydrase; Rubisco; soybean; poplar; stromal protein complex;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The paper provides evidence that in the soluble fraction of buffer-washed soybean and poplar thylakoids the native protein with MW approximately 160 kDa exhibited CA activity. Analysis by SDS-PAGE showed dissociation of 160 kDa protein into a single polypeptide with MW 25 kDa. The reported results can be used in addition to our recent results [(1)] that in pea, one isoenzymic form of soluble chloroplastic CA is associated with Rubisco and, other proteins in an extrinsic multiprotein complex on the outer (stromal) surface of thylakoids. To this conclusion we now added that the aggregate is widespread and its size showed some species-specific variation.
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页码：621 / 626
页数：6
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