Rapamycin enhances IFN-γ and IL-4 production in co-culture of gδ T and dendritic cells from mice with lipopolysaccharide-induced acute lung injury

被引:4
|
作者
Chen, X. L. [1 ]
Li, J. [2 ]
Xu, G. G. [2 ]
Li, H. X. [2 ]
Guo, J. [2 ,3 ]
机构
[1] Capital Med Univ, Beijing Shijitan Hosp, Dept Gerontol, Beijing, Peoples R China
[2] Chinese Peoples Liberat Army Gen Hosp, Med Sch Chinese PLA, Nanlou Resp Dis Dept, Beijing, Peoples R China
[3] Tsinghua Univ, Med Ctr, Beijing Tsinghua Changgung Hosp, Dept Resp Med, Beijing, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
g delta T cell; Dendritic cell; LPS-induced lung injury model; ELISA; RT-PCR; INDUCED INFLAMMATION; IMMUNE-RESPONSES; CARCINOMA; DIFFERENTIATION; RECOGNITION; LYMPHOCYTES; MACROPHAGES; INFECTION; SURVIVAL; INNATE;
D O I
10.4238/gmr.15027511
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study aimed to study the role of rapamycin (RAPA) in modulating the interaction between g delta T cells and dendritic cells (DCs) in a lipopolysaccharide (LPS)-induced acute lung injury mouse model. Mice were injected with LPS to establish the acute lung injury model or LPS + RAPA to assess the role of RAPA in modulating cell interactions. Mice were injected with PBS or RAPA alone as controls. g delta T cells and DCs were isolated from all mice and assessed by flow cytometry and fluorescence microscopy. The isolated g delta T cells and DCs were cultured independently or co-cultured to study their interactions. Enzyme-linked immunosorbent assay was performed to assess the expression of the cytokines, namely, interferon (IFN)-gamma, interleukin (IL)-4, tumor necrosis factor (TNF)-alpha and IL-12 in the individually cultured or co-cultured g delta T cells and DCs, and reverse transcription-polymerase chain reaction (RT-PCR) was employed to investigate the levels of relevant mRNAs. Our study found that co-culture of g delta T cells and DCs from mice treated with LPS + RAPA have reduced expression of IFN-gamma and IL-4 (but not TNF-alpha and IL-12) compared to mice treated with LPS only. These results were confirmed by RT-PCR, where the levels of IFN-gamma and IL-4 mRNA were also reduced. This study may provide useful information in understanding the interaction between g delta T cells and DCs in the LPS-induced lung injury model in mice.
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页数:12
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