Aureimonas psammosilene sp. nov., isolated from the roots of Psammosilene tunicoides

One motile strain designated, YIM DR1026(T) was isolated from the roots of Psammosilene tunicoides collected from Gejiu, Yunnan province, China. The cells of strain YIM DR1026(T) were Gram-negative and short-rod shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM DR1026(T) was a member of the genus Aureimonas and closely related to Aureimonas rubiginis (96.7%). DNA-DNA relatedness values between strain YIM 1026(T) and Aureimonas rubiginis BCRC 80440(T) was 38.2 +/- 1.5%. The ANI value between YIM DR1026(T) and other Aureimonas members were below the cut-off level (95-96%) recommended as the average nucleotide identity (ANI) criterion for interspecies identity. Strain YIM DR1026(T) grew at 4-30 degrees C (optimum 28 degrees C), pH 4.0-9.0 (optimum pH 6.0-7.0) and tolerated NaCl (w/v) up to 1% (optimum 0%). Q-10 was sole the respiratory ubiquinone present in YIM DR1026(T). Polar lipids of strain YIM DR1026(T) were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, unidentified aminolipid and unidentified polar lipid. The genomic G + C content was 64.6 mol%. The major fatty acids were C-18:1 omega 7c, C-16:0 and summed feature 3 (C(16:1 omega)7c/C(16:1 omega)6c). Based on phenotypic, phylogenetic, chemotaxonomic and genome comparison, strain YIM DR1026(T) represents a novel species of the genus Aureimonas, for which the name Aureimonas psammosilene sp. nov. is proposed. The type strain is YIM DR1026(T) (= KCTC 42691(T) = NBRC 112412(T)).