Development and characterization of microsatellite markers for the endangered Chinese yew Taxus chinensis var. mairei (Taxaceae)

被引：6
|
作者：
Xue, H. [1 ,2 ]
Lu, C. H. [1 ]
Wu, X. B. [2 ]
机构：
[1] Nanjing Forestry Univ, Coll Forest Resources & Environm, Nanjing, Jiangsu, Peoples R China
[2] Anhui Normal Univ, Coll Life Sci, Wuhu, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Taxus chinensis var. mairei; Microsatellite; Polymorphism; POLYMORPHISMS;
D O I：
10.4238/2012.May.14.3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We isolated and characterized 11 microsatellite loci for the Chinese yew, Taxus chinensis var. mairei, an endangered tree species in China, by constructing a (CA)(12)-enriched library. The number of alleles per locus ranged from 5 to 10. The observed heterozygosities ranged from 0.2500 to 0.8333 and the expected heterozygosities ranged from 0.5196 to 0.8680. No significant linkage disequilibrium was detected at these loci. However, four loci significantly deviated from Hardy-Weinberg equilibrium. The null alleles were found to be present at locus Tach9 and locus Tach11 by the Micro-checker test (P < 0.001). These polymorphic loci could be employed in research of gene flow and spatial genetic patterns of T. chinensis var. mairei.
引用
收藏
页码：1296 / 1299
页数：4
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