Crystallization and preliminary X-ray analysis of the unliganded recombinant catalytic subnnit of cAMP-dependent protein kinase

X-ray diffraction-quality crystals of the unliganded mouse recombinant catalytic subunit of cAMP-dependent protein kinase were grown by the hanging-drop vapour-diffusion technique using 2-methyl-2,4-pentanediol as precipitant. The crystals belong to the monoclinic space group P2(1) with unit-cell parameters a = 48.9, b = 147.4, c = 54.2 Angstrom, beta = 110.2 degrees. A data set to 3.0 Angstrom resolution with 92% completeness has been collected using synchrotron radiation. The unit cell contains four molecules of molecular weight 40 kDa with a corresponding volume solvent content of 45%.