"In vitro" capacitation and subsequent acrosome reaction are related to changes in the expression and location of midpiece actin and mitofusin-2 in boar spermatozoa

被引：12
|
作者：
Ramio-Lluch, L. [1 ]
Fernandez-Novell, J. M. [2 ]
Pena, A. [1 ]
Bucci, D. [3 ]
Rigau, T. [1 ]
Rodriguez-Gil, J. E. [1 ]
机构：
[1] Autonomous Univ Barcelona, Sch Vet Med, Dept Anim Med & Surg, Bellaterra, Spain
[2] Univ Barcelona, Dept Biochem & Mol Biol, Barcelona, Spain
[3] Univ Bologna, Sch Vet Med, DIMORFIPA, Ozzano Delle Emilia, Italy
来源：
THERIOGENOLOGY | 2012年 / 77卷 / 05期
关键词：
Boar sperm; Capacitation; Acrosome reaction; Mitochondria; Actin; Mitofusin-2; MAMMALIAN SPERM CAPACITATION; TYROSINE PHOSPHORYLATION; PROTEIN-PHOSPHORYLATION; MITOCHONDRIAL-FUNCTION; MEMBRANE; FERTILITY; MOTILITY; DISEASE; JC-1;
D O I：
10.1016/j.theriogenology.2011.10.004
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The induction of "in vitro" capacitation (IVC) and subsequent, progesterone-induced "in vitro" acrosome reaction (IVAR) was concomitant with an increase in actin polymerization, also showing an increase in actin presence at the apical area of the midpiece. The presence of mitofusin-2, a protein involved in the regulation of the coordinated mitochondrial function, expanded from midpiece to the principal piece after IVC and IVAR. All of these results indicate that the increase of boar sperm mitochondrial activity during IVC and the first minutes of IVAR is concomitant with changes in the expression and location of both actin and mitofusin-2. Our results suggest that both actin and mitofusin-2 play important roles in the modulation of boar sperm mitochondrial function, both by originating changes in the protein membrane environment and by changes in the mitochondrial structure itself. (C) 2012 Elsevier Inc. All rights reserved.
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页码：979 / 988
页数：10
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