Specific and Rapid Quantification of Viable Listeria monocytogenes Using Fluorescence in situ Hybridization in Combination with Filter Cultivation

被引：15
|
作者：
Fuchizawa, Ikufumi [1 ]
Shimizu, Shigemasa [1 ]
Ootsubo, Masashi [2 ]
Kawai, Yuji [1 ]
Yamazaki, Koji [1 ]
机构：
[1] Hokkaido Univ, Fac Fisheries Sci, Div Marine Life Sci, Hakodate, Hokkaido 0418611, Japan
[2] Hokkaido Ind Technol Ctr, Dept Res & Dev, Hakodate, Hokkaido 0410801, Japan
来源：
MICROBES AND ENVIRONMENTS | 2009年 / 24卷 / 03期
关键词：
Listeria monocytogenes; fluorescence in situ hybridization; specific detection; FISHFC; 16S RIBOSOMAL-RNA; ACCUMULATING ORGANISMS; VIBRIO-HARVEYI; MESSENGER-RNA; PCR; ENUMERATION; PROBE; FISH; FOOD; WATER;
D O I：
10.1264/jsme2.ME09102
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We developed a new method that rapidly and specifically enumerates only viable Listeria monocytogenes in food using fluorescence in situ hybridization in combination with filter cultivation (FISHFC). Viable L. monocytogenes could be specifically quantified within 16 h using an Alexa647-labeled mRL-2 probe. The coefficient of the correlation between the new method and the conventional plating method was 0.959.
引用
收藏
页码：273 / 275
页数：3
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