Production of herbicide-resistant sweet potato plants transformed with the bar gene

被引：29

作者：

Yi, Gibum ^{[1
]}

Shin, Young-Mi ^{[1
]}

Choe, Goh ^{[1
]}

Shin, Byoungchul ^{[1
]}

Kim, Young Soon ^{[1
]}

Kim, Kyung-Moon ^{[1
]}

机构：

[1] Chonnam Natl Univ, Kumho Life & Environm Sci Lab, Kwangju 500712, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 04期

关键词：

basta; biolistics; Chlorophenol Red; Ipomoea batatas (L.) Lam; transformation;

D O I：

10.1007/s10529-006-9278-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Herbicide-resistant sweet potato plants were produced through biolistics of embryogenic calli derived from shoot apical meristems. Plant materials were bombarded with the vectors containing the beta-glucuronidase gene (gusA) and the herbicide-resistant gene (bar). Selection was carried out using phosphinothricin (PPT). Transformants were screened by the histochemical GUS and Chlorophenol Red assays. PCR and Southern-blot analyses indicated the presence of introduced bar gene in the genomic DNA of the transgenic plants. When sprayed with Basta, the transgenic sweet potato plants was tolerant to the herbicide. Hence, we report successful transformation of the bar gene conferring herbicide resistance to sweet potato.

引用

页码：669 / 675

页数：7

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