Design and application of a microfluidic cell lysis microelectrode chip

被引:8
|
作者
Jiang, Feng [1 ,2 ]
Chen, Jianjun [2 ,3 ]
Yu, Jun [2 ]
机构
[1] Cent South Univ Forestry & Technol, Sch Comp & Informat Engn, Changsha 410004, Hunan, Peoples R China
[2] Huazhong Univ Sci & Technol, Sch Opt & Elect Informat, Wuhan 430074, Peoples R China
[3] Huazhong Agr Univ, Coll Sci, Wuhan, Peoples R China
基金
中国国家自然科学基金;
关键词
Cell lysis; microelectrode chip; microfluidic; POST-GENOME ERA; DC VOLTAGE; DEVICE; ELECTROPORATION;
D O I
10.1080/10739149.2015.1076839
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Microelectrode chips were designed and prepared for cell lysis. The method involved pulverization of the cells by an electrical signal and attachment on the chip. Cell lysis was performed by a 10 volt square wave. This is a reduced voltage compared with traditional methods. This design offers an improved lysing rate, simple operation and design, and low cost.
引用
收藏
页码:223 / 232
页数:10
相关论文
共 50 条
  • [1] Integrated microfluidic cell culture and lysis on a chip
    Nevill, J. Tanner
    Cooper, Ryan
    Dueck, Megan
    Breslauer, David N.
    Lee, Luke P.
    LAB ON A CHIP, 2007, 7 (12) : 1689 - 1695
  • [2] Single cell injection and lysis on a microfluidic chip
    Gao, J
    Yin, XF
    Fang, ZL
    Xia, FQ
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2003, 24 (09): : 1582 - 1584
  • [3] Single cell injection and lysis on a microfluidic chip
    Gao, Jian
    Yin, Xue-Feng
    Fang, Zhao-Lun
    Xia, Fang-Quan
    Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities, 2003, 24 (09): : 1582 - 1584
  • [4] Design, simulation and application of multichannel microfluidic chip for cell migration
    Li, Huilai
    Yang, Xiao
    Wu, Xiaosong
    Li, Zhigang
    Hong, Chenggang
    Liu, Yong
    Zhu, Ling
    Yang, Ke
    Shengwu Yixue Gongchengxue Zazhi/Journal of Biomedical Engineering, 2022, 39 (01): : 128 - 138
  • [5] A cell electrofusion microfluidic chip with micro-cavity microelectrode array
    Ning Hu
    Xiaoling Zhang
    Jun Yang
    Sang W. Joo
    Shizhi Qian
    Microfluidics and Nanofluidics, 2013, 15 : 151 - 160
  • [6] A cell electrofusion microfluidic chip with micro-cavity microelectrode array
    Hu, Ning
    Zhang, Xiaoling
    Yang, Jun
    Joo, Sang W.
    Qian, Shizhi
    MICROFLUIDICS AND NANOFLUIDICS, 2013, 15 (02) : 151 - 160
  • [7] Fabrication of a novel liquid metal microelectrode in microfluidic chip
    Gong, Yanli
    Peng, Bei
    Weng, Xuan
    Jiang, Hai
    MODERN PHYSICS LETTERS B, 2021, 35 (27):
  • [8] Microfluidic chip design with application in biochemistry using polymers
    Esinenco, Dorin
    Rebigan, Roxana
    CAS 2005: INTERNATIONAL SEMICONDUCTOR CONFERENCE, 2005, 1-2 : 409 - 412
  • [9] Integration of single cell injection, cell lysis, separation and detection of intracellular constituents on a microfluidic chip
    Gao, J
    Yin, XF
    Fang, ZL
    LAB ON A CHIP, 2004, 4 (01) : 47 - 52
  • [10] Integrated electrical concentration and lysis of cells in a microfluidic chip
    Church, Christopher
    Zhu, Junjie
    Huang, Guohui
    Tzeng, Tzuen-Rong
    Xuan, Xiangchun
    BIOMICROFLUIDICS, 2010, 4 (04):