Flocculent activity of a recombinant protein from Moringa oleifera Lam. seeds

被引：74
|
作者：
Broin, M
Santaella, C
Cuine, S
Kokou, K
Peltier, G
Joët, T
机构：
[1] Univ Lome, Fac Sci, Lab Bot & Ecol Vegetale, Lome, Togo
[2] Univ Mediterranee, CEA Cadarache,Direct Sci Vivant, UMR 163 CNRS,Dept Ecophysiol Vegetale & Microbiol, Lab Ecol Microbienne & Rhizophere, F-13108 St Paul Les Durance, France
[3] Univ Mediterranee, CEA Cadarache 1000,Direct Sci Vivant, UMR 163 CNRS,Dept Ecophysiol Vegetale & Microbiol, Lab Ecophysiol & Photosynth, F-13108 St Paul Les Durance, France
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2002年 / 60卷 / 1-2期
关键词：
D O I：
10.1007/s00253-002-1106-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Seeds of the tropical tree Moringa oleifera contain small storage proteins able to flocculate particles in suspension in water. The cDNA encoding one of these flocculent proteins, MO2.1, was cloned and the recombinant protein was expressed in Escherichia coli. The flocculent activity of the purified recombinant MO2.1 was assayed on clays and bacteria using light and confocal microscopy and GFP-overexpressing bacteria. We show that MO2.1, is able to aggregate montmorillonite clay particles as well as gram-positive and gram-negative bacteria. We discuss the use of recombinant proteins to study flocculating properties and improve water purification processes.
引用
收藏
页码：114 / 119
页数：6
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