Identification of S-nitrosylated proteins in postmortem pork muscle using modified biotin switch method coupled with isobaric tags

被引：12

作者：

Liu, Rui ^{[1
]}

Fu, Qingquan ^{[1
,2
]}

Lonergan, Steven ^{[3
]}

Huff-Lonergan, Elisabeth ^{[3
]}

Xing, Lujuan ^{[1
]}

Zhang, Lili ^{[1
]}

Bai, Yun ^{[1
]}

Zhou, Guanghong ^{[1
]}

Zhang, Wangang ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Jiangsu Collaborat Innovat Ctr Meat Prod & Proc Q, Minist Educ China,Key Lab Meat Proc & Qual Contro, Nanjing 210095, Jiangsu, Peoples R China

[2] Nanjing Xiaozhuang Univ, Sch Biochem & Environm Engn, Nanjing 211171, Jiangsu, Peoples R China

[3] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA

来源：

MEAT SCIENCE | 2018年 / 145卷

基金：

中国国家自然科学基金;

关键词：

Longissimus thomcis; S-nitrosylation; biotin switch assay; isobaric tags; NITRIC-OXIDE SYNTHASE; MEAT TENDERNESS; INHIBITION; OXIDATION; DENITROSYLATION; QUANTIFICATION; MECHANISMS; BIOMARKERS; RELEASE; BOVINE;

D O I：

10.1016/j.meatsci.2018.07.027

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The objective of this study was to identify the S-nitrosylated proteins in aging samples of pork longissimus thoracis muscle (aged 0 and 3 d) and to study the effects of exogenous S-nitrosoglutathione (GSNO, concentration at 10 and 100 mu M) treatments of aged 0 d sample. After validating modified biotin switch method, the samples were labeled with tandem mass tags (TMT126.129) for the LC-MS/MS analysis. A total of 366 peptides were identified to be S-nitrosylated corresponding to 339 proteins. Comparison of total intensity and individual S-nitrosylated sites between aging samples revealed that S-nitrosylation did occur in pork muscle during postmortem aging through possible pathways of denitrosylation and transnitrosylation. GSNO treatment groups showed a considerable number of potential cysteines could be modified with high thiol-reactivity. It was deduced that S-nitrosylation could be involved in the postmortem metabolic process possibly through the regulation of activity or function of glycolytic enzymes, calcium release, heat shock proteins, antioxidant enzymes and myofibrillar proteins.

引用

页码：431 / 439

页数：9

共 7 条

[1] Proteome profiling of s-nitrosylated synaptosomal proteins by isobaric mass tags
Wijasa, Teodora Stella
Sylvester, Marc
Brocke-Ahmadinejad, Nahal
Kummer, Markus P.
Brosseron, Frederic
Gieselmann, Volkmar
Heneka, Michael T.
JOURNAL OF NEUROSCIENCE METHODS, 2017, 291 : 95 - 100
[2] A bioinformatics study on characteristics, metabolic pathways, and cellular functions of the identified S-nitrosylated proteins in postmortem pork muscle
Liu, Rui
Zhang, Chaoyang
Xing, Lujuan
Zhang, Lili
Zhou, Guanghong
Zhang, Wangang
FOOD CHEMISTRY, 2019, 274 : 407 - 414
[3] Proteomics identification of differential S-nitrosylated proteins between the beef with intermediate and high ultimate pH using isobaric iodoTMT switch assay
Zhu, Qiongniu
Xing, Lujuan
Hou, Qin
Liu, Rui
Zhang, Wangang
MEAT SCIENCE, 2021, 172
[4] A "fluorescence switch" technique increases the sensitivity of proteomic detection and identification of S-nitrosylated proteins
Tello, Daniel
Tarin, Carlos
Ahicart, Patricia
Breton-Romero, Rosa
Lamas, Santiago
Martinez-Ruiz, Antonio
PROTEOMICS, 2009, 9 (23) : 5359 - 5370
[5] Proteomic Quantification and Site-Mapping of S-Nitrosylated Proteins Using Isobaric iodoTMT Reagents
Qu, Zhe
Meng, Fanjun
Bomgarden, Ryan D.
Viner, Rosa I.
Li, Jilong
Rogers, John C.
Cheng, Jianlin
Greenlief, C. Michael
Cui, Jiankun
Lubahn, Dennis B.
Sun, Grace Y.
Gu, Zezong
JOURNAL OF PROTEOME RESEARCH, 2014, 13 (07) : 3200 - 3211
[6] Detergent-free biotin switch combined with liquid chromatography/tandem mass spectrometry in the analysis of S-nitrosylated proteins
Han, Pewei
Chen, Chang
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2008, 22 (08) : 1137 - 1145
[7] A RAPID APPROACH FOR THE DETECTION, QUANTIFICATION, AND DISCOVERY OF NOVEL SULFENIC ACID OR S-NITROSOTHIOL MODIFIED PROTEINS USING A BIOTIN-SWITCH METHOD
Burgoyne, Joseph R.
Eaton, Philip
METHODS IN ENZYMOLOGY, VOL 473: THIOL REDOX TRANSITIONS IN CELL SIGNALING, PT A: CHEMISTRY AND BIOCHEMISTRY OF LOW MOLECULAR WEIGHT AND PROTEIN THIOLS, 2010, 473 : 281 - 303

← 1 →