Comparative Cytogenetics of Giant Trahiras Hoplias aimara and H. intermedius (Characiformes, Erythrinidae): Chromosomal Characteristics of Minor and Major Ribosomal DNA and Cross-Species Repetitive Centromeric Sequences Mapping Differ among Morphologically Identical Karyotypes

被引：17

作者：

Blanco, D. R. ^{[2
]}

Lui, R. L. ^{[2
]}

Vicari, M. R. ^{[1
]}

Bertollo, L. A. C. ^{[2
]}

Moreira-Filho, O. ^{[2
]}

机构：

[1] Univ Estadual Ponta Grossa, Lab Citogent & Evolucao, Dept Biol Estrutural Mol & Genet, BR-84030900 Ponta Grossa, PR, Brazil

[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP, Brazil

来源：

CYTOGENETIC AND GENOME RESEARCH | 2011年 / 132卷 / 1-2期

基金：

巴西圣保罗研究基金会;

关键词：

Chromosome banding; Cot-1; DNA; Cross-species probing; FISH mapping; FISH cytotaxonomy; Hoplias lacerdae group; rDNA; SATELLITE DNA; ORGANIZER REGIONS; 5S RDNA; FISH; MALABARICUS; FLUORESCENCE; TELEOSTEI; PISCES; HETEROCHROMATIN; LOCALIZATION;

D O I：

10.1159/000320923

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Karyotype and cytogenetic characteristics of 2 species of giant trahiras, Hoplias intermedius, Sao Francisco river basin, and Hoplias aimara, Arinos river (Amazon basin), were examined by conventional (C-banding, Ag-NOR, DAPI/CMA(3) double-staining) and fluorescent in situ hybridization (FISH) with 5S, 18S rDNA probes and cross-species Cot-1 DNA probing. Both species invariably had diploid chromosome number 2n = 50 and identical karyotypes composed of 10 pairs of metacentric and 15 pairs of submetacentric chromosomes. On the other hand, staining with base-specific fluorochromes (CMA(3), DAPI) and FISH mapping of repetitive DNA sequences showed extensive interspecific differences: while the genome of H. aimara had one submetacentric pair bearing CMA(3)-positive (DAPI-negative) sites, that of H. intermedius had 4 such pairs; while FISH with a 5S rDNA probe showed one (likely homologous) signal-bearing pair, that with 18S rDNA displayed one signal-bearing pair in H. intermedius and 2 such pairs in H. aimara. Cross-species FISH probing with Cot-1 DNA prepared from total DNA of both species showed no signals of Cot-1 DNA from H. aimara on chromosomes of H. intermedius but reciprocally (Cot-1 DNA from H. intermedius on chromosomes of H. aimara) displayed signals on at least 4 chromosome pairs. Present findings indicate (i) different composition of repetitive sequences around centromeres, (ii) different NOR phenotypes and (iii) distinct taxonomic status of both giant trahira species. Copyright (C) 2010 S. Karger AG, Basel

引用

页码：71 / 78

页数：8