Molecular markers associated with antimicrobial resistance and genotypes of Campylobacter jejuni and Campylobacter coli isolated from broiler and swine flocks in southeast Brazil

被引：0
|
作者：
Dias, Thomas Salles [1 ,3 ]
Costa, Gisllany Alves [1 ]
Figueira, Arthur de Almeida [1 ]
Machado, Leandro dos Santos [1 ]
da Cunha, Nathalie Costa [2 ]
do Nascimento, Elmiro Rosendo [1 ]
Pereira, Virginia Leo de Almeida [1 ,2 ]
de Aquino, Maria Helena Cosendey [1 ,2 ]
机构：
[1] Univ Fed Fluminense, Fac Vet, Postgrad Program Vet Med Vet Hyg & Proc Technol An, Niteroi, RJ, Brazil
[2] Univ Fed Fluminense, Fac Vet, Dept Prevent Vet Med, Niteroi, RJ, Brazil
[3] Fac Vet, Rua Vital Brasil Filho 64, BR-24230340 Niteroi, Brazil
来源：
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2022年 / 88卷
关键词：
Antimicrobial resistance; Campylobacter; Genotypes; FLUOROQUINOLONE RESISTANCE; ERYTHROMYCIN RESISTANCE; ANTIBIOTIC-RESISTANCE; PCR ASSAY; STRAINS; SPP; MUTATIONS; POULTRY; GENE; SUSCEPTIBILITIES;
D O I：
10.1016/j.cimid.2022.101866
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
This study aimed to identify molecular markers associated with antimicrobial resistance and genotype isolates of Campylobacter spp. from broiler and swine flocks due to its importance to one-health. C. jejuni (n=27) and C. coli (n = 35) strains were screened for the antimicrobial genetic markers C257T in gyrA, A2074C and A2075G in 23S rRNA, CmeABC, ermB, tetO and blaOXA61 by PCR. Fifteen strains had SVR-flaA and porA genes sequenced to evaluate their genetic diversity. Among C. jejuni strains 62.96% had C257T mutation and only one strain had A2075G mutation. CmeA, cmeB, cmeC, tetO and blaOXA61 were detected respectively in 92.59%, 100%, 100%, 85.19%, 85.19% of the strains. All C. coli had C257T mutation; 48.75% had A2075G and cmeA, cmeB, cmeC, tetO, blaOXA61 were detected in 8.57%, 94.29%, 91.43%, 91.43%, 80%, respectively. Twelve porA and SVR-flaA alleles were detected, with a Simpson index of diversity value of 0.962.
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页数：6
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