Development and characterization of 14 polymorphic microsatellite loci in the endangered tree Euptelea pleiospermum (Eupteleaceae)

被引：12
|
作者：
Zhang, J. [1 ,2 ]
Yao, X. [1 ]
Wei, X. [1 ,2 ]
Chen, L. [1 ]
Jiang, M. [1 ]
机构：
[1] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
MOLECULAR ECOLOGY RESOURCES | 2008年 / 8卷 / 02期
基金：
澳大利亚研究理事会;
关键词：
conservation genetics; Euptelea pleiospermum; Eupteleaceae; microsatellite;
D O I：
10.1111/j.1471-8286.2007.01939.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fourteen polymorphic microsatellite loci were developed and characterized for the endangered and tertiary relict tree, Euptelea pleiospermum. A genomic DNA enrichment protocol was used to isolate microsatellite loci and polymorphism was explored using 32 individuals from one natural population. The observed number of alleles ranged from two to nine. The ranges of observed and expected heterozygosities were 0.25-1.00 and 0.22-0.85, respectively. These microsatellite markers provide powerful tools for the ongoing conservation genetic studies of E. pleiospermum.
引用
收藏
页码：314 / 316
页数：3
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