SHIP2 controls PtdIns(3,4,5)P3 levels and PKB activity in response to oxidative stress

Reactive oxygen species (ROS) are known to be involved in redox signalling pathways that may contribute to normal cell function as well as disease progression. The tumour suppressor PTEN and the inositol 5-phosphatase SHIP2 are critical enzymes in the control of Ptdlns(3,4,5)P-3 level. It has been reported that oxidants, including those produced in cells such as macrophages, can activate downstream signalling via the inactivation of PTEN. The present study evaluates the potential impact of SHIP2 on phosphoinositides in cells exposed to sodium peroxide. We used a model of SHIP2 deficient mouse embryonic fibroblasts (MEFs) stimulated by H2O2: at 15 min, PtdIns(3,4,5)P-3 was markedly increased in SHIP2 -/- cells as compared to +/+ cells. In contrast, no significant increase in Ptdlns(3,4)P-2 could be detected at 15 or 120 min incubation of the cells with H2O2 (0.6 mM). PKB activity was also upregulated in SHIP2 -/- cells as compared to +/+ cells in response to H2O2. SHTP2 add back experiments in SHIP2 -/- cells confirm its critical role as a lipid phosphatase in the control of Ptdlns(3,4,5)P-3 level in response to H2O2. We conclude that SHIP2 lipid phosphatase activity plays an important role in the metabolism PtdIns(3,4,5)P-3 which is demonstrated in oxygen stressed cells. (c) 2007 Elsevier Inc. All rights reserved.