Comparison of real-time reverse-transcription loop-mediated isothermal amplification and real-time reverse-transcription polymerase chain reaction for detection of noroviruses in municipal wastewater

被引：13
|
作者：
Suzuki, Yoshihiro [1 ]
Narimatsu, Shogo [1 ]
Furukawa, Takashi [1 ]
Iwakiri, Akira [2 ]
Miura, Miho [2 ]
Yamamoto, Shogo [2 ]
Katayama, Hiroyuki [3 ]
机构：
[1] Miyazaki Univ, Fac Engn, Dept Civil & Environm Engn, Miyazaki 8892192, Japan
[2] Miyazaki Prefectural Inst Publ Hlth & Environm, Microbiol Lab, Miyazaki 8892155, Japan
[3] Univ Tokyo, Sch Engn, Dept Urban Engn, Bynkyo Ku, Tokyo 1138656, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2011年 / 112卷 / 04期
关键词：
Noroviruses; Real-time RT-LAMP; Real-time RT-PCR; Monitoring; Wastewater; Sensitivity; VIRAL GASTROENTERITIS; ENTERIC VIRUSES; OUTBREAK; RIVER;
D O I：
10.1016/j.jbiosc.2011.06.012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The monitoring of NVs in municipal wastewater by both real-time RT-IAMP and real-time RT-PCR, and the comparison of these two methods with respect to NV detection were carried out. The change in NVs detected by real-time RT-LAMP agreed well with that detected by real-time RT-PCR. In contrast, the correlation between the copy number determined by real-time RT-PCR and the threshold time (Tt) determined by real-time RT-LAMP obtained during monitoring was not significant (0.1<p) for both NV-GI and NV-GII. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：369 / 372
页数：4
相关论文
共 50 条
  • [1] Comparison of reverse-transcription loop-mediated isothermal amplification and reverse-transcription polymerase chain reaction for grass carp reovirus
    Yang, Shuixian
    Wang, Kai-Yu
    Yang, Zexiao
    [J]. ACTA VETERINARIA BRNO, 2015, 84 (03) : 215 - 223
  • [2] Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus
    Yan, Liping
    Peng, Shan
    Yan, Pixi
    Zhou, Jiewen
    Teng, Qiaoyang
    Li, Guoxin
    Li, Xuesong
    Li, Zejun
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2012, 182 (1-2) : 50 - 55
  • [3] Real-Time Reverse-Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus
    Peyrefitte, Christophe N.
    Boubis, Laetitia
    Coudrier, Daniel
    Bouloy, Michele
    Grandadam, Marc
    Tolou, Hugues J.
    Plumet, Sebastien
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (11) : 3653 - 3659
  • [4] Real-time detection of mRNA splicing variants with specifically designed reverse-transcription loop-mediated isothermal amplification
    Su, Fengxia
    Wang, Guanhao
    Ji, Jianing
    Zhang, Pengbo
    Wang, Fangfang
    Li, Zhengping
    [J]. RSC ADVANCES, 2020, 10 (11) : 6271 - 6276
  • [5] Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction
    Corman, V. M.
    Eckerle, I.
    Bleicker, T.
    Zaki, A.
    Landt, O.
    Eschbach-Bludau, M.
    van Boheemen, S.
    Gopal, R.
    Ballhause, M.
    Bestebroer, T. M.
    Muth, D.
    Mueller, M. A.
    Drexler, J. F.
    Zambon, M.
    Osterhaus, A. D.
    Fouchier, R. M.
    Drosten, C.
    [J]. EUROSURVEILLANCE, 2012, 17 (39): : 3 - 8
  • [6] A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of human astrovirus
    Wei, Haiyan
    Zeng, Jing
    Deng, Congliang
    Zheng, Chengzhong
    Zhang, Ximeng
    Ma, Dan
    Yi, Yong
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2013, 188 (1-2) : 126 - 131
  • [7] Evaluation of real-time reverse-transcription loop-mediated isothermal amplification assay for clinical diagnosis of West Nile virus in patients
    Kumar, Jyoti S.
    Saxena, Divyasha
    Parida, Manmohan
    Rathinam, Sivakumar
    [J]. INDIAN JOURNAL OF MEDICAL RESEARCH, 2018, 147 : 293 - 298
  • [8] Development of a multiplex real-time quantitative reverse-transcription polymerase chain reaction for the detection of four bee viruses
    Iredale, Marley E.
    Cobb, Galen
    Vu, Emily D.
    Ghosh, Saptarshi
    Ellis, James D.
    Bonning, Bryony C.
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2024, 328
  • [9] Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus
    Parida, M
    Posadas, G
    Inoue, S
    Hasebe, F
    Morita, K
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (01) : 257 - 263
  • [10] Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assays for rhinovirus detection
    Nakauchi, Mina
    Takayama, Ikuyo
    Takahashi, Hitoshi
    Semba, Shohei
    Saito, Shinji
    Kubo, Hideyuki
    Kaida, Atsushi
    Oba, Kunihiro
    Nagata, Shiho
    Odagiri, Takato
    Kageyama, Tsutomu
    [J]. JOURNAL OF MEDICAL VIROLOGY, 2019, 91 (07) : 1232 - 1238
12345