Design, 3D printing and validation of a novel low-cost high-capacity sitting-drop bridge for protein crystallization

被引:3
|
作者
Talapatra, Sandeep K. [1 ,2 ]
Penny, Matthew R. [1 ]
Hilton, Stephen T. [1 ]
Kozielski, Frank [1 ]
机构
[1] UCL, Sch Pharm, Dept Pharmaceut & Biol Chem, 29-39 Brunswick Sq, London WC1N 1AX, England
[2] AstraZeneca, IMED Biotech Unit, Alderley Pk, London, England
关键词
protein crystallization; sitting-drop crystallization; hanging-drop crystallization; vapour-diffusion crystallization; microbridges; 3D printing;
D O I
10.1107/S1600576718017545
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Sitting-drop protein crystallization is not used as commonly as the hanging-drop method for crystal optimization owing to the limitations of commercially available sitting-drop bridges, particularly when they are used in conjunction with 24-well crystallization plates. The commercially available sitting-drop bridge, containing space for only a single drop, restricts their wider use. Proteins that preferentially crystallize under sitting-drop conditions therefore require more work, time and resources for their optimization. In response to these limitations, and using 3D printing, a new sitting-drop bridge has been designed and developed, where five crystallization drops can be placed simultaneously in each well of a 24-well crystallization plate. This significantly simplifies the process and increases the potential of sitting drops in crystal optimization, reducing costs and hence overcoming the limitations of current approaches.
引用
收藏
页码:171 / 174
页数:4
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