Cloning and Identification of MicroRNAs in Earthworm (Eisenia fetida)

被引：6
|
作者：
Huang, Xue-Mei [1 ]
Tian, Qing-Nan [1 ]
Bao, Zhen-Xia [1 ]
Qin, Yun-Fei [1 ]
Chen, San-Jun [1 ]
Lu, Ping [1 ]
Zhang, Xiao-Ling [1 ]
Zhang, Yi-Zhe [1 ]
Zhang, Shou-Tao [1 ,2 ]
机构：
[1] Zhengzhou Univ, Dept Bioengn, Zhengzhou 450001, Henan, Peoples R China
[2] Zhengzhou Univ, Mobile Stn Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
来源：
BIOCHEMICAL GENETICS | 2012年 / 50卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
miRNA; piRNA; Eisenia fetida; Regeneration; SMALL RNAS; LET-7; EXPRESSION; PIWI; REGENERATION; GERMLINE; SEQUENCE; SYSTEM; PCR;
D O I：
10.1007/s10528-011-9452-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs (miRNAs) (noncoding RNAs of 20-25 nucleotides) play important roles in the post-transcriptional regulation of gene expression in various eukaryotes and prokaryotes. Piwi-interacting RNAs function by combining with PIWI proteins to regulate protein synthesis and to stabilize mRNA, the chromatin framework, and genome structure. This study investigates the role of miRNAs in regeneration. A scrDNA library was constructed, and 17 noncoding RNAs from Eisenia fetida (an optimal model for the study of earthworm regeneration) were cloned and characterized. In addition, reverse transcription polymerase chain reaction was performed to analyze the expression of four small RNAs during different developmental stages. The expression levels of these RNAs in regenerating tissue were higher than in normal tissue, and the expression patterns of these small RNAs were unique during development.
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页码：1 / 11
页数：11
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