This research was designed to understand heterosexual men's interpersonal reactions toward a gay male individual and to examine how threat and pre-existing antigay prejudice impact these encounters. In one experiment, we manipulated the ostensible sexual orientation of an assigned work partner and assessed participants' perceptions of threat indirectly, using a measure of psychological distancing. Results revealed that, regardless of antigay prejudice, participants psychologically distanced more from the gay male than from the heterosexual male. In the second experiment, we manipulated threat and the sexual orientation of the work partner to examine aggressive responding toward the work partner. Participants exposed to a threat to their masculinity behaved more aggressively toward the gay work partner, regardless of their level of antigay prejudice.