Crystallization and preliminary X-ray analysis of LC3-I

Aut7/Apg8 is located in the intermediate structures of the autophagosome and plays an essential role in autophagosome formation. The processed form, cleaved at a C-terminus of Gly120 and called LC3-I, was expressed, purified and crystallized in two crystal forms. One form belongs to space group I4(1), with unit-cell parameters a = 84.39, c = 36.89 Angstrom. The other form belongs to space group P4(1) or P4(3), with unit-cell parameters a = 60.48, c = 35.28 Angstrom. From the latter form, a complete diffraction data set was collected to 2.1 Angstrom resolution.