The retroviral proteinase active site and the N-terminus of Ddi1 are required for repression of protein secretion

被引：21

作者：

White, Rhian E. ^{[1
]}

Dickinson, J. Richard ^{[1
]}

Semple, Colin A. M. ^{[2
]}

Powell, David J. ^{[3
]}

Berry, Colin ^{[1
]}

机构：

[1] Cardiff Univ, Cardiff Sch Biosci, Cardiff CF10 3AT, S Glam, Wales

[2] MRC, Human Genet Unit, Inst Genet & Mol Med, Edinburgh EH4 2XU, Midlothian, Scotland

[3] Glaxo SmithKline, Med Res Ctr, Stevenage SG1 2NY, Herts, England

来源：

FEBS LETTERS | 2011年 / 585卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Saccharomyces; Ddi1; VSM1; Protein secretion; Proteinase; Ubiquitin; Aspartic; DAMAGE-INDUCIBLE PROTEINS; HIDDEN MARKOV-MODELS; CELL-CYCLE CONTROL; STRUCTURE PREDICTION; UBIQUITIN RECEPTOR; UBA DOMAINS; YEAST; REGULATOR; HOMOLOGY; BINDING;

D O I：

10.1016/j.febslet.2010.11.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Ddi1 protein of the yeast Saccharomyces cerevisiae is involved in numerous interactions with the ubiquitin system, which may be mediated by its N-terminal ubiquitin like domain and its C-terminal ubiquitin associated domain. Ddi1 also contains a central region with all the features of a retroviral aspartic proteinase, which was shown to be important in cell-cycle control. Here we demonstrate an additional role for this domain, along with the N-terminal region, in protein secretion. These results further substantiate the hypothesis that Ddi1 functions in vivo as a catalytically-active aspartic proteinase. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：139 / 142

页数：4

共 50 条

[1] Ddi1, a eukaryotic protein with the retroviral protease fold
Sirkis, Roy
Gerst, Jeffrey E.
Fass, Deborah
JOURNAL OF MOLECULAR BIOLOGY, 2006, 364 (03) : 376 - 387
[2] The nonconserved N-terminus of protein phosphatases 1 influences its active site
Xie, XiuJie
Huang, Wei
Xue, ChengZhe
Wei, Qun
BMB REPORTS, 2008, 41 (12) : 881 - 885
[3] Exploiting Protein N-Terminus for Site-Specific Bioconjugation
De Rosa, Lucia
Di Stasi, Rossella
Romanelli, Alessandra
D'Andrea, Luca Domenico
MOLECULES, 2021, 26 (12):
[4] The ClpP N-terminus coordinates substrate access with protease active site reactivity
Jennings, Laura D.
Bohon, Jen
Chance, Mark R.
Licht, Stuart
BIOCHEMISTRY, 2008, 47 (42) : 11031 - 11040
[5] MYELIN BASIC-PROTEIN BINDS GTP AT A SINGLE SITE IN THE N-TERMINUS
CHAN, KC
RANWANI, J
MOSCARELLO, MA
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 152 (03) : 1468 - 1473
[6] Ddi1-like protein from Leishmania major is an active aspartyl proteinase
María J. Perteguer
Paulino Gómez-Puertas
Carmen Cañavate
Francehuli Dagger
Teresa Gárate
Elizabeth Valdivieso
Cell Stress and Chaperones, 2013, 18 : 171 - 181
[7] Ddi1-like protein from Leishmania major is an active aspartyl proteinase
Perteguer, Maria J.
Gomez-Puertas, Paulino
Canavate, Carmen
Dagger, Francehuli
Garate, Teresa
Valdivieso, Elizabeth
CELL STRESS & CHAPERONES, 2013, 18 (02): : 171 - 181
[8] Two Distinct Sites on Gβγ are Required for Binding to the N-Terminus Versus the Activation Site on Adenylyl Cyclase
Brand, Cameron S.
Sadana, Rachna
Malik, Sundeep
Smrcka, Alan V.
Dessauer, Carmen W.
FASEB JOURNAL, 2011, 25
[9] The extreme N-terminus of the Caulobacter crescentus surface-layer protein directs export of passenger proteins from the cytoplasm but is not required for secretion of the native protein
Bingle, WH
Le, KD
Smit, J
CANADIAN JOURNAL OF MICROBIOLOGY, 1996, 42 (07) : 672 - 684
[10] The N-terminus of SECIS Binding Protein 2 is Required for Processive Selenocystine Incorporation in Selenoprotein P
Pinkerton, Mark Hurst
Vetick, Michael
Shetty, Sumangala P.
Copeland, Paul R.
FASEB JOURNAL, 2017, 31

← 1 2 3 4 5 →