Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy

被引：90
|
作者：
Shiku, H [1 ]
Matsue, T [1 ]
Uchida, I [1 ]
机构：
[1] TOHOKU UNIV,GRAD SCH ENGN,DEPT APPL CHEM,SENDAI,MIYAGI 98077,JAPAN
来源：
ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 07期
关键词：
D O I：
10.1021/ac950824c
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as similar to 10(4) CEA molecules in a single 20-mu m-radius spot.
引用
收藏
页码：1276 / 1278
页数：3
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