The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

被引:10
|
作者
Brunello, Giulia [1 ,2 ]
Becker, Kathrin [3 ]
Scotti, Luisa [1 ,4 ]
Drescher, Dieter [3 ]
Becker, Juergen [1 ]
John, Gordon [1 ]
机构
[1] Univ Clin Dusseldorf, Dept Oral Surg, D-40225 Dusseldorf, Germany
[2] Univ Padua, Dept Neurosci, I-35128 Padua, Italy
[3] Univ Clin Dusseldorf, Dept Orthodont, D-40225 Dusseldorf, Germany
[4] Dent Practice, D-46147 Oberhausen, Germany
关键词
antiseptic; bone; cetylpyridinium chloride; chlorhexidine; mouthrinse; peri-implantitis; periodontitis; PERI-IMPLANTITIS TREATMENT; OCTENIDINE MOUTH RINSE; SURGICAL-TREATMENT; HUMAN FIBROBLASTS; EPITHELIAL-CELLS; CONSENSUS REPORT; DOUBLE-BLIND; DECONTAMINATION; EFFICACY; AGENTS;
D O I
10.3390/ijms22189986
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several decontamination methods for removing biofilm from implant surfaces during surgical peri-implantitis treatment have been reported, including the intraoperative usage of chlorhexidine (CHX)-based antiseptics. There is a lack of information on possible adverse effects on bone healing. The study aimed to examine the impact of three CHX-based mouthwashes on osteoblast-like cells (SaOS-2) in vitro. Cells were cultured for three days in 96-well binding plates. Each well was randomly treated for either 30, 60 or 120 s with 0.05% CHX combined with 0.05% cetylpyridinium chloride (CPC), 0.1% CHX, 0.2% CHX or sterile saline (NaCl) as control. Cell viability, cytotoxicity and apoptosis were assessed at day 0, 3 and 6. Cell viability resulted in being higher in the control group at all time points. At day 0, the CHX 0.2 group showed significantly higher cytotoxicity values compared to CHX 0.1 (30 s), CHX + CPC (30 s, 60 s and 120 s) and control (60 s and 120 s), while no significant differences were identified between CHX + CPC and both CHX 0.1 and NaCl groups. All test mouthwashes were found to induce apoptosis to a lower extent compared to control. Results indicate that 0.2% CHX presented the highest cytotoxic effect. Therefore, its intraoperative use should be carefully considered.
引用
收藏
页数:12
相关论文
共 50 条
  • [1] Pueraria thunbergiana promotes differentiation and mineralization in human osteoblast-like SaOS-2 cells.
    Kim, DY
    Huh, JE
    Yang, HR
    Baek, YH
    Lee, JD
    Park, DS
    Yoo, MC
    JOURNAL OF BONE AND MINERAL RESEARCH, 2005, 20 (09) : S243 - S243
  • [2] Osteogenic Potential of Biosilica on Human Osteoblast-Like (SaOS-2) Cells
    Matthias Wiens
    Xiaohong Wang
    Ute Schloßmacher
    Ingo Lieberwirth
    Gunnar Glasser
    Hiroshi Ushijima
    Heinz C. Schröder
    Werner E. G. Müller
    Calcified Tissue International, 2010, 87 : 513 - 524
  • [3] Osteogenic Potential of Biosilica on Human Osteoblast-Like (SaOS-2) Cells
    Wiens, Matthias
    Wang, Xiaohong
    Schlossmacher, Ute
    Lieberwirth, Ingo
    Glasser, Gunnar
    Ushijima, Hiroshi
    Schroeder, Heinz C.
    Mueller, Werner E. G.
    CALCIFIED TISSUE INTERNATIONAL, 2010, 87 (06) : 513 - 524
  • [4] The role of surface implant treatments on the biological behavior of SaOS-2 osteoblast-like cells. An in vitro comparative study
    Conserva, Enrico
    Menini, Maria
    Ravera, Giambattista
    Pera, Paolo
    CLINICAL ORAL IMPLANTS RESEARCH, 2013, 24 (08) : 880 - 889
  • [5] Effect of "Uraemic Toxins" on the proliferation of Human Osteoblast-Like Cells (SaOS-2) in vitro.
    Picton, ML
    Hutchison, AJ
    Gokal, R
    Hoyland, JA
    KIDNEY INTERNATIONAL, 1999, 55 (05) : 2107 - 2108
  • [6] Effect of ''uraemic toxins'' on the proliferation of human osteoblast-like cells (SaOS-2) in vitro.
    Picton, ML
    Hutchison, AJ
    Gokal, R
    Hoyland, JA
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 1997, 8 : A2582 - A2582
  • [7] Effect of Three Chlorhexidine-Based Mouthwashes on Human Gingival Fibroblasts: An In Vitro Study
    Brunello, Giulia
    Becker, Kathrin
    Scotti, Luisa
    Drescher, Dieter
    Becker, Juergen
    John, Gordon
    APPLIED SCIENCES-BASEL, 2022, 12 (05):
  • [8] Adhesion of human osteoblast-like cells (Saos-2) to carbon nanotube sheets
    Akasaka, Tsukasa
    Yokoyama, Atsuro
    Matsuoka, Makoto
    Hashimoto, Takeshi
    Abe, Shigeaki
    Uo, Motohiro
    Watari, Fumio
    BIO-MEDICAL MATERIALS AND ENGINEERING, 2009, 19 (2-3) : 147 - 153
  • [9] Puerariae radix promotes differentiation and mineralization in human osteoblast-like SaOS-2 cells
    Huh, JE
    Yang, HR
    Park, DS
    Choi, DY
    Baek, YH
    Cho, EM
    Cho, YJ
    Kang-Il, K
    Kim, DY
    Lee, JD
    JOURNAL OF ETHNOPHARMACOLOGY, 2006, 104 (03) : 345 - 350
  • [10] Adenosine Triphosphate stimulates differentiation and mineralization in human osteoblast-like Saos-2 cells
    Cutarelli, Alessandro
    Marini, Mario
    Tancredi, Virginia
    D'Arcangelo, Giovanna
    Murdocca, Michela
    Frank, Claudio
    Tarantino, Umberto
    DEVELOPMENT GROWTH & DIFFERENTIATION, 2016, 58 (04) : 400 - 408