Quantitative analysis of thuringiensin by micellar electrokinetic capillary chromatography

A method for quantitative analysis of thuringiensin is established using micellar electrokinetic capillary chromatography (MEKC). Tryptophan was used as an internal standard. The running buffer contained phosphate-borate (20 mM) and sodium dodecyl sulfate (100 nM) with 10% acetonitrile. Electrophoresis was conducted under a voltage of 15 kV for 20 min. The peak area ratio of thuringiensin to tryptophan was calculated From the resultant electropherogram. Results indicated a high correlation between the concentration of thuringiensin and the peak area ratio of thuringiensin to tryptophan. The general equation of linear regression line is very similar to the equation obtained from HPLC results established previously in our laboratory. However, MEKC is a faster, simpler and less expensive assay than the traditional HPLC method. (C) 1998 Elsevier Science B.V. All rights reserved.