Human and minipig cytochrome P450 2E1

被引：0
|
作者：
Baranová, J [1 ]
Anzenbacherová, E [1 ]
Zuber, R [1 ]
Anzenbacher, P [1 ]
Soucek, P [1 ]
机构：
[1] Palacky Univ, Inst Pharmacol, CR-77147 Olomouc, Czech Republic
来源：
CYTOCHROMES P450, BIOCHEMISTRY, BIOPHYSICS AND DRUG METABOLISM | 2003年
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cytochrome P450 2E1 (CYP2E1) from minipig liver microsomes was isolated and characterized. It exhibits high structural and functional similarity to the human orthologous form. In the reconstituted system, it metabolizes the two prototypic substrates of human CYP2E1, chlorzoxazone and p-nitrophenol. Moreover, the diethyldithiocarbamate, used as a characteristic inhibitor of the human CYP2E1 activity, inhibits the minipig CYP2E1 enzyme effectively. Hence, the minipig enzyme exhibits the properties of the CYP2E1 and may serve as a good model of its human counterpart.
引用
收藏
页码：143 / 147
页数：5
相关论文
共 50 条
  • [1] Minipig cytochrome P450 2E1:: Comparison with human enzyme
    Baranová, J
    Anzenbacherová, E
    Anzenbacher, P
    Soucek, P
    [J]. DRUG METABOLISM AND DISPOSITION, 2005, 33 (06) : 862 - 865
  • [2] Genetic polymorphism of human cytochrome P450 2E1
    Nedelcheva, V
    Persson, I
    IngelmanSundberg, M
    [J]. CYTOCHROME P450, PT B, 1996, 272 : 218 - 225
  • [3] Human Cytochrome P450 2E1 metabolizes acrylamide to glycidamide
    Settels, Eva
    Bernauer, Ulrike
    Palavinskas, Richard
    Klaffke, Horst Stefan
    Gundert-Remy, Ursula
    Appel, Klaus Erich
    [J]. TOXICOLOGY LETTERS, 2007, 172 : S108 - S109
  • [4] Direct electrochemistry of immobilized human cytochrome P450 2E1
    Fantuzzi, A
    Fairhead, M
    Gilardi, G
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (16) : 5040 - 5041
  • [5] Measurement of immunoreactive cytochrome P450 2E1 in human leucocytes
    Scobbie, AE
    Mason, HJ
    [J]. BIOMARKERS, 1999, 4 (04) : 311 - 317
  • [6] Active site topology of human cytochrome P450 2E1
    Mackman, R
    Guo, ZY
    Guengerich, FP
    OrtizdeMontellano, PR
    [J]. CHEMICAL RESEARCH IN TOXICOLOGY, 1996, 9 (01) : 223 - 226
  • [7] Inactivation of cytochrome P450 2E1 by capsaicin
    Smeal, SJ
    Reilly, CA
    Yost, GS
    [J]. FASEB JOURNAL, 2002, 16 (05): : A940 - A941
  • [8] Stereoselective metabolism of enflurane by human liver cytochrome P450 2E1
    Garton, KJ
    Yuen, P
    Meinwald, J
    Thummel, KE
    Kharasch, ED
    [J]. DRUG METABOLISM AND DISPOSITION, 1995, 23 (12) : 1426 - 1430
  • [9] The Role of Human Cytochrome P450 2E1 in Liver Inflammation and Fibrosis
    Xu, Jun
    Ma, Hsiao-Yen
    Liang, Shuang
    Sun, Mengxi
    Karin, Gabriel
    Koyama, Yukinori
    Hu, Ronglin
    Quehenberger, Oswald
    Davidson, Nicholas O.
    Dennis, Edward A.
    Kisseleva, Tatiana
    Brenner, David A.
    [J]. HEPATOLOGY COMMUNICATIONS, 2017, 1 (10) : 1043 - 1057
  • [10] CHLORINATED QUINOLINES AS FLUORESCENT PROBES OF HUMAN CYTOCHROME P450 2E1
    Cui, Xinhe
    Mizutani, Takaharu
    Saeki, Ken-ichi
    [J]. DRUG METABOLISM REVIEWS, 2007, 39 : 253 - 253
12345