Induction of immune responses and prevention of alveolar bone loss by intranasal administration S of mice with Porphyromona gingivanlis fimbriae and recombinant cholera toxin B subunit

被引:10
|
作者
Takahashi, Y.
Kumada, H.
Hamada, N.
Hiashima, Y.
Ozono, S.
Isaka, M.
Yasuda, Y.
Tochikubo, K.
Umemoto, T.
机构
[1] Kanagawa Dent Coll, Dept Oral Microbiol, Yokosuka, Kanagawa 2388580, Japan
[2] Natl Inst Hlth Sci, Div Med Devices, Setagaya Ku, Tokyo 158, Japan
[3] Kanagawa Dent Coll, Inst Frontier Oral Sci, Kanagawa, Japan
[4] Nagoya City Univ, Sch Med, Dept Microbiol, Mizuho Ku, Nagoya, Aichi 467, Japan
[5] Kinjo Gakuin Univ Coll Pharm, Dept Pharm, Moriyama Ku, Nagoya, Aichi, Japan
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 2007年 / 22卷 / 06期
关键词
cholera toxin B subunit; oral bone loss; periodontitis; Porphyromonas gingivalis; vaccine;
D O I
10.1111/j.1399-302X.2007.00373.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Introduction: Adult periodontitis is initiated by specific periodontal pathogens represented by Porphyromonas gingivalis; however, an effective measure for preventing the disease has not yet been established. In this study, the effectiveness of a vaccine composed of fimbriae of P. gingivalis and recombinant cholera toxin B subunit (rCTB) was evaluated using BALB/c mice. Methods: Fimbriae and rCTB were co-administered intranasally to BALB/c mice on days 0, 143 21, and 28. On day 35, mice were sacrificed to determine immunoglobulin levels in serum, saliva, and nasal and lung extracts by enzyme-linked immunosorbent assay. The prevention effect of the vaccine on P. gingivalis-induced periodontitis in mice was evaluated by measuring alveolar bone loss. Results: The rCTB significantly increased serum immunoglobulin (Ig)A levels when mice were administered with a minimal amount (0.5 mu g) of the fimbrial antigen. The adjuvant effect on serum IgG production was indistinct because the minimal amount of the antigen still induced a large amount of IgG. In contrast to systemic responses, a fimbria-specific secretory IgA response was strongly induced by co-administration of rCTB and 0.5 mu g fimbriae, the same amount of the antigen alone scarcely induced a response. Histopathological examination revealed IgA-positive plasma cells in the nasal mucosal tissue but no observable mast cells in the area. In addition, nasal administration of the fimbrial vaccine significantly protected the mice from R gingivalis-mediated alveolar bone loss. Conclusion: Nasal vaccination with a combination of fimbriae and rCTB can be an effective means of preventing P gingivalis-mediated periodontitis.
引用
收藏
页码:374 / 380
页数:7
相关论文
共 41 条
  • [1] Prevention of alveolar bone resorption by intranasal administration of Porphyromonas gingivalis fimbriae and recombinant cholera toxin B subunit in mice.
    Takahashi, Y.
    Kumada, H.
    Hamada, N.
    Ozono, S.
    Haishima, Y.
    Isaka, M.
    Yasuda, Y.
    Tochikubo, K.
    Umemoto, T.
    JOURNAL OF DENTAL RESEARCH, 2003, 82 : B281 - B281
  • [2] Induction of mucosal and systemic immune responses by intranasal immunization using recombinant cholera toxin B subunit as an adjuvant
    Wu, HY
    Russell, MW
    VACCINE, 1998, 16 (2-3) : 286 - 292
  • [3] Local and systemic immune responses to rectal administration of recombinant cholera toxin B subunit in humans
    Jertborn, M
    Nordström, I
    Kilander, A
    Czerkinsky, C
    Holmgren, J
    INFECTION AND IMMUNITY, 2001, 69 (06) : 4125 - 4128
  • [4] Induction and distribution of intestinal immune responses after administration of recombinant cholera toxin B subunit in the ileal pouches of colectomized patients
    Kilhamn, J
    Brevinge, H
    Quiding-Järbrink, M
    Svennerholm, AM
    Jertborn, M
    INFECTION AND IMMUNITY, 2001, 69 (05) : 3466 - 3471
  • [5] Mucosal and systemic antibody responses against an acellular pertussis vaccine in mice after intranasal co-administration with recombinant cholera toxin B subunit as an adjuvant
    Isaka, M
    Yasuda, Y
    Taniguchi, T
    Kozuka, S
    Matano, K
    Maeyama, J
    Morokuma, K
    Ohkuma, K
    Goto, N
    Tochikubo, K
    VACCINE, 2003, 21 (11-12) : 1165 - 1173
  • [6] Intranasal sensitization of Japanese cedar pollen by the co-administration of low doses of cholera toxin but not its recombinant B subunit to mice
    Hirai, T
    Hashiguchi, S
    Torigoe, N
    Toda, Y
    Ito, Y
    Sugimura, K
    MICROBIOLOGY AND IMMUNOLOGY, 2000, 44 (04) : 259 - 266
  • [7] Adjuvant effects of cholera toxin b subunit on immune response to recombinant thyrotropin receptor in mice
    Fan, JL
    Peterson, JW
    Prabhakar, BS
    JOURNAL OF AUTOIMMUNITY, 2000, 14 (01) : 43 - 52
  • [8] Intranasal administration of recombinant Neisseria gonorrhoeae transferrin binding proteins A and B conjugated to the cholera toxin B subunit induces systemic and vaginal antibodies in mice
    Price, GA
    Russell, MW
    Cornelissen, CN
    INFECTION AND IMMUNITY, 2005, 73 (07) : 3945 - 3953
  • [9] Induction and distribution of intestinal immune responses after administration of recombinant cholera toxin B subunit in the ileal pouches of colectomized patients (vol 69, pg 3466, 2001)
    Kilhamn, J
    Brevinge, H
    Quiding-Järbrink, M
    Svennerholm, AM
    Jertborn, M
    INFECTION AND IMMUNITY, 2001, 69 (10) : 6564 - 6564
  • [10] Induction of immune response and prevention of alveolar bone loss with recombinant Porphyromonas gingivalis peptidylarginine deiminase
    Zhu, Chunhui
    Yang, Jin
    Sun, Junyi
    Shi, Jianfeng
    Gou, Jianzhong
    Li, Ang
    ARCHIVES OF ORAL BIOLOGY, 2013, 58 (12) : 1777 - 1783