Two experiments with broiler chicks and one experiment with laying hens were conducted to determine the MEn value of conjugated linoleic acid (CLA). In Experiment 1, for 8 d, 16-d-old chicks were fed diets in which 4, 8, or 12% of CLA Source A or 4, 8, or 12% of soybean oil (SO) was substituted for glucose. Dietary MEn increased linearly (P less than or equal to 0.001) with increments of CLA Source A or SO. Regression analysis relating increases in dietary MEn and increments of the dietary fat sources showed that the MEn values of CLA Source A and SO, when evaluated separately, were 7,419 and 8,429 kcal/ kg, respectively. In Experiment 2, feed was withheld from laying hens for 38 h and then the hens were force-fed diets containing 15% glucose, 15% CLA Source A, or 15% SO (two feedings of 30 g each). Excreta samples were collected for 36 h after the last feeding. The ME, values obtained for CLA Source A and SO were 8,517 and 8,437 kcal/kg, respectively. The MEn of CLA Source B (higher in unsaturated fatty acids than CLA Source A) was determined in Experiment 3 by feeding diets containing 4, 8, or 12% CLA Source B to 14-d-old chicks. Increases in dietary MEn with increments of CLA Source B were curvilinear, with resulting MEn of 9,375 to 9,588 kcal/kg of fat when CLA Source B was fed at 4 or 8% of the diet and 7,917 kcal/kg when fed at 12% of the diet. Results of this research show that CLA sources can contribute substantial energy to diets, but the MEn value of CLA sources for young chicks varies with fatty acid composition and dietary concentration.