The pYV virulence plasmids of Yersinia pseudotuberculosis and Y. pestis contain a conserved DNA region responsible for the mobilization by the self-transmissible plasmid pYE854

被引：13

作者：

Hammerl, Jens A. ^{[1
]}

Freytag, Barbara ^{[1
]}

Lanka, Erich ^{[2
]}

Appel, Bernd ^{[1
]}

Hertwig, Stefan ^{[1
]}

机构：

[1] Bundesinst Risikobewertung, D-12277 Berlin, Germany

[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany

来源：

ENVIRONMENTAL MICROBIOLOGY REPORTS | 2012年 / 4卷 / 04期

关键词：

ENTEROCOLITICA; SALMONELLA; EVOLUTION; PROTEIN;

D O I：

10.1111/j.1758-2229.2012.00353.x

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Pathogenic Yersinia strains possess a 70 kb virulence plasmid which can be transmitted to other strains by conjugation systems expressed by co-resident plasmids. We isolated a 720 bp fragment of the Yersinia pseudotuberculosis virulence plasmid p1340 that mediated mobilization of the vector pIV2 by the self-transmissible plasmid pYE854. The p1340 mobilization region contains the resolvase gene tnpR and its proposed resolution site res. Both elements are required for mobilization. Plasmid transfer was associated with the formation of co-integrates in which res was fused to pYE854 fragments by short nucleotide stretches similarly present within res. blast searches and PCR experiments revealed the presence of the mobilization region in the virulence plasmids of other Y. pseudotuberculosis and Y. pestis strains but not in pYV of Yersinia enterocolitica.

引用

页码：433 / 438

页数：6