Development, chromosome location and genetic mapping of EST-SSR markers in wheat

被引：30
|
作者：
Chen, HM [1 ]
Li, LZ [1 ]
Wei, XY [1 ]
Li, SS [1 ]
Lei, TD [1 ]
Hu, HZ [1 ]
Wang, HG [1 ]
Zhang, XS [1 ]
机构：
[1] Shandong Agr Univ, Shandong Key Lab Crop Biol, Coll Agron, Tai An 271018, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2005年 / 50卷 / 20期
基金：
中国国家自然科学基金;
关键词：
wheat; EST-SSR; primer; nulli-tetrasome; RIL; genetic map;
D O I：
10.1360/982005-379
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A number of 151695 wheat expression sequence tags (ESTs) that originated from GenBank/dbEST from July 14, 2003 to August 24, 2004 were used to search for simple sequence repeats (SSRs) with motif 2-5 bp, and 2038 simple sequence repeats (EST-SSRs), which accounted for 1.34% of EST database, were identified. Based on these SSR sequences, 249 EST-SSR primer pairs and 166 amplified clear bands in various wheat cultivars were designed. These EST-SSR markers can be used as new molecular markers in wheat and related species. Using Chinese Spring nulli-tetrasomic lines, 93 EST-SSR primer pairs and 193 EST-SSR loci were located on 19 wheat chromosomes except for 4A and 4B. Forty-three loci were mapped on 11 chromosomes of the genetic framework map previously constructed using recombinant inbred lines.
引用
收藏
页码：2328 / 2336
页数：9
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