Genotyping fetal DNA by non-invasive means: extraction from maternal plasma

被引：37

作者：

Nelson, M ^{[1
]}

Eagle, C ^{[1
]}

Langshaw, M ^{[1
]}

Popp, H ^{[1
]}

Kronenberg, H ^{[1
]}

机构：

[1] Royal Prince Alfred Hosp, Inst Haematol, Camperdown, NSW 2050, Australia

来源：

VOX SANGUINIS | 2001年 / 80卷 / 02期

关键词：

DNA; fetal; genotyping; plasma;

D O I：

10.1046/j.1423-0410.2001.00019.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background and Objectives Identification of fetal DNA in maternal plasma may allow genetic analysis without the use of invasive techniques. The aim of this study was to extract DNA from maternal plasma, identify fetal material through the presence of SRY or RHD gene sequences and assess the reliability of these results. Materials and Methods A polymerase chain reaction (PCR) method of a commercial kit was used with primers for SRY or exon 10 of the RHD gene sequence. Results Multiple plasma samples were collected from 60 women who were evaluable for either SRY or RHD, or both, fetally derived DNA sequences. Fetal DNA was present in the plasma throughout the pregnancies and for some hours or days after delivery. Conclusion Fetal DNA can be reliably detected in maternal plasma from early in pregnancy and normally is cleared within days of delivery.

引用

页码：112 / 116

页数：5

共 50 条

[1] Non-invasive fetal RHD and RHCE genotyping from maternal plasma in alloimmunized pregnancies
Hromadnikova, I
Vesela, K
Benesova, B
Nekovarova, K
Duskova, D
Vlk, R
Spalova, I
Gerychova, R
Hakenova, A
Rosenbaumova, Z
Vlasin, P
Vlachova, A
Palasek, V
Roznakova, E
Calda, P
PRENATAL DIAGNOSIS, 2005, 25 (12) : 1079 - 1083
[2] Maternal Circulant Free Fetal DNA for Non-Invasive Antenatal RHD Genotyping
Spaltro, Gabriella
Pizzi, Mara Nicoletta
Fantini, Norma Nadia
Truglio, Francesca
Paccapelo, Cinzia
Zanoni, Irene
Villa, Maria Antonietta
Revelli, Nicoletta
Prati, Daniele
TRANSFUSION, 2018, 58 : 197A - 197A
[3] Non-Invasive Fetal RHD Genotyping by Maternal Plasma in a Racially Mixed Population
Cozac, A. C.
Miyashiro, K.
Silva, C. G.
Pinto, G. N.
Rizzatti, E. G.
TRANSFUSION, 2011, 51 : 39A - 39A
[4] NON-INVASIVE FETAL BLOOD GROUP GENOTYPING WITH DNA FROM MATERNAL PLASMA: A SEVEN-YEAR CLINICAL EXPERIENCE
de Haas, M.
Scheffer, P. G.
van der Schoot, C. E.
Bossers, B. E. M.
Ligthart, P. C.
Schuitemaker, L. D. M.
de Haas, M.
VOX SANGUINIS, 2010, 99 : 24 - 24
[5] Accuracy of non-invasive fetal Rh genotyping from maternal blood
Grotegut, CA
Gaughan, JP
Geifman-Holtzman, O
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2005, 193 (06) : S136 - S136
[6] Non-invasive fetal RHD genotyping and fetal sexing in maternal blood
de Haas, M.
Bossers, B. E. M.
Soussan, A. Ait
Ligthart, P. C.
Schuitemaker, L. D. M.
Page-Christiaens, G. C. M. L.
Van der Schoot, C. E.
VOX SANGUINIS, 2006, 91 : 145 - 145
[7] Non-Invasive Prenatal RHD Genotyping Using Cell-Free Fetal DNA from Maternal Plasma: An Italian Experience
Picchiassi, Elena
Di Renzo, Gian Carlo
Tarquini, Federica
Bini, Vittorio
Centra, Michela
Pennacchi, Luana
Galeone, Fabiana
Micanti, Mara
Coata, Giuliana
TRANSFUSION MEDICINE AND HEMOTHERAPY, 2015, 42 (01) : 22 - 28
[8] Fetal DNA in maternal plasma: towards non-invasive prenatal diagnosis
Lo, YMD
CLINICAL CHEMISTRY, 2001, 47 (02) : 362 - 362
[9] Non-Invasive Prenatal Determination of Fetal RhD Genotyping from Maternal Plasma: A Preliminary Study in Pakistan
Mohammed, Nuruddin
Kakal, Fatima
Somani, Mehreen
Zafar, Wajiha
JCPSP-JOURNAL OF THE COLLEGE OF PHYSICIANS AND SURGEONS PAKISTAN, 2010, 20 (04): : 246 - 249
[10] Non-invasive fetal ABO genotyping in maternal plasma using real-time PCR
Song, Wenqian
Zhou, Shihang
Shao, Linnan
Wang, Ni
Pan, Lingzi
Yu, Weijian
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2015, 53 (12) : 1943 - 1950

← 1 2 3 4 5 →