Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O60

被引：3
|
作者：
Ovchinnikova, Olga G. [1 ]
Kocharova, Nina A. [1 ]
Parkhomchuk, Angelina A. [1 ]
Bialczak-Kokot, Magdalena [2 ]
Shashkov, Alexander S. [1 ]
Knirel, Yuriy A. [1 ]
Rozalski, Antoni [2 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Univ Lodz, Dept Immunobiol Bacteria, Inst Microbiol Biotechnol & Immunol, PL-90237 Lodz, Poland
来源：
CARBOHYDRATE RESEARCH | 2011年 / 346卷 / 02期
关键词：
Providencia alcalifaciens; O-Antigen; Lipopolysaccharide; Bacterial polysaccharide structure; PROTEUS;
D O I：
10.1016/j.carres.2010.11.016
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An O-polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O60 and studied by sugar and methylation analyses as well as (1)H and (13)C NMR spectroscopy, including 2D ROESY and (1)H, (13)C HMBC experiments in D(2)O and a ROESY experiment in a 9:1 H(2)O-D(2)O mixture to reveal correlations for NH protons. It was found that the polysaccharide is built up of linear pentasaccharide repeating units containing an amide of o-glucuronic acid with L-serine and has the following structure: -> 4)-beta-D-Glcp-(1 -> 3)-alpha-D-Galp-(1 -> 4)-beta-D-GalpNAc-(1 -> 4)-beta-D-GlepA-(1 -> 3)-beta-D-GalpNAc-(1 -> L-Ser-(2-6) The O-antigen studied is structurally and serologically closely related to the O-antigen of Proteus vulgaris O44. (c) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：377 / 380
页数：4
相关论文
共 50 条
  • [1] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O48
    Ovchinnikova, Olga G.
    Kocharova, Nina A.
    Shashkov, Alexander S.
    Knirel, Yuriy A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2012, 347 (01) : 168 - 171
  • [2] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O12
    Parkhomchuk, Angelina A.
    Kocharova, Nina A.
    Bialczak-Kokot, Magdalena
    Shashkov, Alexander S.
    Chizhov, Alexander O.
    Knirel, Yuriy A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2010, 345 (09) : 1235 - 1239
  • [3] The structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O30
    Kocharova, NA
    Ovchinnikova, OG
    Torzewska, A
    Shashkov, AS
    Knirel, YA
    Rozalski, A
    CARBOHYDRATE RESEARCH, 2006, 341 (06) : 786 - 790
  • [4] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O28
    Ovchinnikova, Olga G.
    Kocharova, Nina A.
    Kondakova, Anna N.
    Bialczak-Kokot, Magdalena
    Shashkov, Alexander S.
    Knirel, Yuriy A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2011, 346 (16) : 2638 - 2641
  • [5] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O29
    Bushmarinov, Ivan S.
    Ovchinnikova, Olga G.
    Kocharova, Nina A.
    Toukach, Filip V.
    Torzewska, Agnieszka
    Shashkov, Alexander S.
    Knirel, Yurly A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2006, 341 (09) : 1181 - 1185
  • [6] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O33
    Ovchinnikova, Olga G.
    Shashkov, Alexander S.
    Chizhov, Alexander O.
    Moryl, Magdalena
    Rozalski, Antoni
    Knirel, Yuriy A.
    CARBOHYDRATE RESEARCH, 2014, 390 : 67 - 70
  • [7] Structure of the O-polysaccharide of Providencia alcalifaciens O19
    Kocharova, NA
    Maszewska, A
    Zatonsky, GV
    Torzewska, A
    Bystrova, OV
    Shashkov, AS
    Knirel, YA
    Rozalski, A
    CARBOHYDRATE RESEARCH, 2004, 339 (02) : 415 - 419
  • [8] Structure of a colitose-containing O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O6
    Ovchinnikova, Olga G.
    Kocharova, Nina A.
    Wykrota, Marianna
    Shashkov, Alexander S.
    Knirel, Yurly A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2007, 342 (14) : 2144 - 2148
  • [9] New structure for the O-polysaccharide of Providencia alcalifaciens O27 and revised structure for the O-polysaccharide of Providencia stuartii O43
    Ovchinnikova, Olga G.
    Bushmarinov, Ivan S.
    Kocharova, Nina A.
    Toukach, Filip V.
    Wykrota, Marianna
    Shashkov, Alexander S.
    Knirel, Yuriy A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2007, 342 (08) : 1116 - 1121
  • [10] Structure of the O-polysaccharide of Escherichia coli O60
    Perepelov, A. V.
    Naumenko, O. I.
    Senchenkova, S. N.
    Shashkov, A. S.
    Chizhov, A. O.
    Knirel, Yu. A.
    RUSSIAN CHEMICAL BULLETIN, 2018, 67 (11) : 2131 - 2134
12345