Single-molecule quantum-dot fluorescence resonance energy transfer

被引：141
|
作者：
Hohng, S [1 ]
Ha, T [1 ]
机构：
[1] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
来源：
CHEMPHYSCHEM | 2005年 / 6卷 / 05期
关键词：
DNA; fluorescence; FRET; quantum dots; single-molecule studies;
D O I：
10.1002/cphc.200400557
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Colloidal semiconductor quantum dots are promising for single-molecule biological imaging due to their outstanding brightness and photostability. As a proof of concept for single-molecule fluorescence resonance energy transfer (FRET) applications, we measured FRET between a single quantum dot and a single organic fluorophore Cy5. DNA Holliday junction dynamics measured with the quantum dot/Cy5 pair are identical to those obtained with the conventional Cy3/Cy5 pair, that is, conformational changes of individual molecules con be observed by using the quantum dot as the donor.
引用
收藏
页码：956 / 960
页数：5
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