Determination of flavonoids in Daphnis Genkwae Flos by high performance liquid chromatography

In order to evaluate the quality of Daphnis Genkwae Flos, a high performance liquid chromatographic method (HPLC) was developed. Six flavonoid constituents from Daphnis Genkwae Flos, genkwanin 5-O-beta -D-primeveroside (1), genkwanin 5-O-beta -D-glucoside (2), genkwanin (3), potassium apigenin 7-O-beta -D-glucuronate (4), apigenin (5) and tiliroside (6) determined by this simple, rapid and accurate method. Samples were analyzed using a Cosmosil 5C(18)-AR reversed phase column by gradient elution with varied proportion of 1.0 % (v/v) acetic acid and acetonitrile as mobile phase at 254 nm. Sulfamethoxypyridazine was used as the internal standard. Regression equations revealed the linear relationship (correlation coefficients: 0.9994-0.9999) between the peak-area ratios flavonoid to internal standard and concentrations of flavonoids. The recoveries of six flavonoids were between 100.9-106.1%. The relative standard deviations of six flavonoids ranged between 0.49 similar to3.46 % (intraday) and 0.52 similar to4.61 % (interday). The contents of the six flavonoids in sixteen crude drugs of Daphnis Genkwae Flos were 1 0.13 similar to0.40 %, 2 0.02 similar to0.12 %, 3 0.24 similar to0.56 %, 4 0.16 similar to1.19 %, 5 0.30 similar to0.73 % and 6 0.15 similar to0.57 %, respectively.