A ratiometric Raman probe for live- cell imaging of hydrogen sulfide in mitochondria by stimulated Raman scattering

被引：46
|
作者：
Zeng, Chen [1 ]
Hu, Fanghao [1 ]
Long, Rong [1 ]
Min, Wei [1 ]
机构：
[1] Columbia Univ, Dept Chem, 3000 Broadway, New York, NY 10027 USA
来源：
ANALYST | 2018年 / 143卷 / 20期
关键词：
FLUORESCENT-PROBE; MICROSCOPY; H2S;
D O I：
10.1039/c8an00910d
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Stimulated Raman Scattering (SRS) coupled with alkyne tags has been an emerging imaging technique to visualize small-molecule species with high sensitivity and specificity. Here we describe the development of a ratiometric Raman probe for visualizing hydrogen sulfide (H2S) species in living cells as the first alkyne-based sensor for SRS microscopy. This probe uses an azide unit as a selective reactive site, and it targets mitochondria with high specificity. The SRS ratiometric images show a strong response to H2S level changes in living cells.
引用
收藏
页码：4844 / 4848
页数：5
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